id	occurrenceID	measurementType	measurementTypeID	measurementValue	measurementValueID	measurementUnit	measurementUnitID	measurementRemarks
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_dloop_R1.fq.gz	001_Win_12_Chlamax_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253255	SAMN51253255			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_dloop_R2.fq.gz	001_Win_12_Chlamax_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax	001_Win_12_Chlamax			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_DL	001_Win_12_Chlamax_01_DL			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.751552795	1.751552795	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59093	5.59093	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.105	0.105	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.013	0.013	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.277	0.277	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	0.956	0.956	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.3	2.3	nanograms per microlitre		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGCGTATAG	CCGCGTATAG			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGGTTCCTA	CCGGTTCCTA			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	13:50:00	13:50:00			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.4056	16.4056	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T13:50-07:00	2022-10-13T13:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.03382833	34.03382833			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.03382833	34.03382833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.0563317	-121.0563317	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	24	24	meters		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28933	28933	reads		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.0563317	-121.0563317			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2419	33.2419	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	383193	383193	reads		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01 | 001_Win_12_Chlamax_02 | 001_Win_12_Chlamax_03	001_Win_12_Chlamax_01 | 001_Win_12_Chlamax_02 | 001_Win_12_Chlamax_03	decimal degrees		
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	113	113			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; CalCOFI-specific station where CTD was cast		80.7 59.9	80.7 59.9			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_025	GEM_R_dLoop3_025			
CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:001_Win_12_Chlamax_01_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01-1_dloop_R1.fq.gz	002_Win_2_90m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253283	SAMN51253283			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01-1_dloop_R2.fq.gz	002_Win_2_90m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m	002_Win_2_90m			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01-1_DL	002_Win_2_90m_01-1_DL			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.351485149	1.351485149	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2712	6.2712	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.216	20.216	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.052	0.052	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.667	1.667	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.101	19.101	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.99	0.99	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GCCGCACTCT	GCCGCACTCT			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGAGGTCGGA	CGAGGTCGGA			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7061	10.7061	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	90	90	meters		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	1875	1875	reads		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7244	33.7244	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	8669	8669	reads		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01 | 002_Win_2_90m_03	002_Win_2_90m_01 | 002_Win_2_90m_03	decimal degrees		
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	15	15			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_029	GEM_R_dLoop1_029			
CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:002_Win_2_90m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_dloop_R1.fq.gz	002_Win_6_10m_02-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253301	SAMN51253301			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_dloop_R2.fq.gz	002_Win_6_10m_02-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m	002_Win_6_10m			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_DL	002_Win_6_10m_02-2_DL			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.872871737	1.872871737	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.69646	5.69646	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.41	0.41	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.299	0.299	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.089	1.089	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.5	8.5	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P267D	P267D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGTAATCGAC	TGTAATCGAC			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGGTGATC	CGCGGTGATC			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.4305	15.4305	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	93569	93569	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3539	33.3539	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	627142	627142	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	decimal degrees		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58601	58601			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_134	GEM_R_dLoop2_134			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_dloop_R1.fq.gz	002_Win_6_10m_02-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253301	SAMN51253301			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_dloop_R2.fq.gz	002_Win_6_10m_02-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m	002_Win_6_10m			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-2_DL	002_Win_6_10m_02-2_DL			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.872871737	1.872871737	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.69646	5.69646	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.41	0.41	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.299	0.299	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.089	1.089	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.5	8.5	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P267D	P267D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGTAATCGAC	TGTAATCGAC			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGGTGATC	CGCGGTGATC			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.4305	15.4305	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	93569	93569	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3539	33.3539	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	627142	627142	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	decimal degrees		
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58601	58601			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_134	GEM_R_dLoop2_134			
CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	CalCOFI_Intercal:DL:002_Win_6_10m_02-2_DL_occ_eb71f3702a62d326e49f1e71264d2ffdbcd6e97c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_dloop_R1.fq.gz	002_Win_6_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253302	SAMN51253302			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_dloop_R2.fq.gz	002_Win_6_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m	002_Win_6_10m			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_DL	002_Win_6_10m_02-3_DL			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.872871737	1.872871737	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.69646	5.69646	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.41	0.41	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.299	0.299	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.089	1.089	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.5	8.5	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P267D	P267D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACAATCT	GTAACAATCT			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTATACAGAG	GTATACAGAG			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.4305	15.4305	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	40639	40639	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3539	33.3539	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	132421	132421	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	decimal degrees		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	27476	27476			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_134	GEM_R_dLoop3_134			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_09c9589da6f3f77b9d0d9774c3b65ce22b5048fe	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_dloop_R1.fq.gz	002_Win_6_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253302	SAMN51253302			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_dloop_R2.fq.gz	002_Win_6_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m	002_Win_6_10m			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_02-3_DL	002_Win_6_10m_02-3_DL			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.872871737	1.872871737	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.69646	5.69646	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.41	0.41	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.299	0.299	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.089	1.089	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.5	8.5	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P267D	P267D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACAATCT	GTAACAATCT			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTATACAGAG	GTATACAGAG			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.4305	15.4305	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	40639	40639	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3539	33.3539	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	132421	132421	reads		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	002_Win_6_10m_01 | 002_Win_6_10m_02 | 002_Win_6_10m_03	decimal degrees		
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	27476	27476			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_134	GEM_R_dLoop3_134			
CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	CalCOFI_Intercal:DL:002_Win_6_10m_02-3_DL_occ_fbfde21bfbd200f6fe20f54f800704515d54122a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_dloop_R1.fq.gz	003_GEM_3_100m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253093	SAMN51253093			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_dloop_R2.fq.gz	003_GEM_3_100m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m	003_GEM_3_100m			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_DL	003_GEM_3_100m-1_DL			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.31044	6.31044	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.2	0.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GACTGAGTAG	GACTGAGTAG			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACTATCAAC	CACTATCAAC			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.4144	10.4144	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	164245	164245	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	636088	636088	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2229	2229			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_009	GEM_R_dLoop1_009			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_dloop_R1.fq.gz	003_GEM_3_100m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253093	SAMN51253093			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_dloop_R2.fq.gz	003_GEM_3_100m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m	003_GEM_3_100m			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-1_DL	003_GEM_3_100m-1_DL			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.31044	6.31044	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.2	0.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GACTGAGTAG	GACTGAGTAG			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACTATCAAC	CACTATCAAC			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.4144	10.4144	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	164245	164245	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	636088	636088	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2229	2229			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_009	GEM_R_dLoop1_009			
CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	CalCOFI_Intercal:DL:003_GEM_3_100m-1_DL_occ_f46765cd3ba5cf8ba790cc5e26844a7d683dda1d	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-2_dloop_R1.fq.gz	003_GEM_3_100m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253094	SAMN51253094			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-2_dloop_R2.fq.gz	003_GEM_3_100m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m	003_GEM_3_100m			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m-2_DL	003_GEM_3_100m-2_DL			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.31044	6.31044	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.2	0.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGTCTCGT	TCAGTCTCGT			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAATCCGTG	ATAATCCGTG			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.4144	10.4144	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	123654	123654	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	710055	710055	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8087	8087			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_009	GEM_R_dLoop2_009			
CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m-2_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m_dloop_R1.fq.gz	003_GEM_3_100m_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253092	SAMN51253092			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m_dloop_R2.fq.gz	003_GEM_3_100m_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m	003_GEM_3_100m			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_3_100m_DL	003_GEM_3_100m_DL			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.31044	6.31044	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.2	0.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGTAACGTT	ATGTAACGTT			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.4144	10.4144	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	75873	75873	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	315144	315144	reads		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	147	147			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_009	GEM_R_dLoop3_009			
CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	CalCOFI_Intercal:DL:003_GEM_3_100m_DL_occ_b238fd2cb524b7b65f63687dea7d0a2754668c83	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_08997f3ed812fa7cf9b53e6a2ae666a7bf0d7799	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_0e15290ee1ddfd6394d7ef7763ec7bc414acc1d0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_11c3ba98ecba42187cd583e3173c8c95a397a3ac	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_2b17ac23feb39c9a46ed2095a00aba9dc2eee18b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_4590568b8e028f6d5b764b4a4f85cdf2392a4fde	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_49d73c0300c192a0f95a02bf2e42303b5e87e899	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_537bc184b49d393e9e9f389d1014979b65898dde	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_65e65dba1772abb7cf5053d804925eb499a63e02	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_8aba2ad052d464341db28870ab3782a098ed77c8	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ab1550ec33bf7df4aca27544318dd436c9b058d2	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_aed3a79766c24a30de2b8a13bfedc1e3727e76d5	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_bfd6b70dc178265ffd7d1728185ed72cc5fc2e0e	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R1.fq.gz	003_GEM_7_10m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253100	SAMN51253100			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_dloop_R2.fq.gz	003_GEM_7_10m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m	003_GEM_7_10m			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_GEM_7_10m-2_DL	003_GEM_7_10m-2_DL			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89562	5.89562	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.08	0.08	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCTCAATACC	TCTCAATACC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCGAACGTC	CGCGAACGTC			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:12:00	12:12:00			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.6942	13.6942	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:12-07:00	2022-10-14T12:12-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	397473	397473	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	870459	870459	reads		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	395809	395809			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_007	GEM_R_dLoop2_007			
CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:003_GEM_7_10m-2_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R1.fq.gz	003_Win_4_100m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253307	SAMN51253307			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R2.fq.gz	003_Win_4_100m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_DL	003_Win_4_100m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGTGTATG	ACAGTGTATG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGTATGTTC	CCGTATGTTC			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	134599	134599	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	640134	640134	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3126	3126			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_032	GEM_R_dLoop1_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R1.fq.gz	003_Win_4_100m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253307	SAMN51253307			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R2.fq.gz	003_Win_4_100m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_DL	003_Win_4_100m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGTGTATG	ACAGTGTATG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGTATGTTC	CCGTATGTTC			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	134599	134599	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	640134	640134	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3126	3126			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_032	GEM_R_dLoop1_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R1.fq.gz	003_Win_4_100m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253307	SAMN51253307			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R2.fq.gz	003_Win_4_100m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_DL	003_Win_4_100m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGTGTATG	ACAGTGTATG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGTATGTTC	CCGTATGTTC			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	134599	134599	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	640134	640134	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3126	3126			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_032	GEM_R_dLoop1_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_34bda6cd01ec08505a80a113b3204cc617f9ea07	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R1.fq.gz	003_Win_4_100m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253307	SAMN51253307			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_dloop_R2.fq.gz	003_Win_4_100m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-1_DL	003_Win_4_100m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGTGTATG	ACAGTGTATG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGTATGTTC	CCGTATGTTC			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	134599	134599	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	640134	640134	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3126	3126			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_032	GEM_R_dLoop1_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R1.fq.gz	003_Win_4_100m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253308	SAMN51253308			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R2.fq.gz	003_Win_4_100m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_DL	003_Win_4_100m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTGGACAAGT	GTGGACAAGT			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AAGGCCACGG	AAGGCCACGG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	97574	97574	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	854221	854221	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1417	1417			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_032	GEM_R_dLoop2_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R1.fq.gz	003_Win_4_100m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253308	SAMN51253308			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R2.fq.gz	003_Win_4_100m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_DL	003_Win_4_100m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTGGACAAGT	GTGGACAAGT			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AAGGCCACGG	AAGGCCACGG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	97574	97574	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	854221	854221	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1417	1417			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_032	GEM_R_dLoop2_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R1.fq.gz	003_Win_4_100m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253308	SAMN51253308			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_dloop_R2.fq.gz	003_Win_4_100m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01-2_DL	003_Win_4_100m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTGGACAAGT	GTGGACAAGT			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AAGGCCACGG	AAGGCCACGG			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	97574	97574	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	854221	854221	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1417	1417			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_032	GEM_R_dLoop2_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:003_Win_4_100m_01-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_dloop_R1.fq.gz	003_Win_4_100m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253306	SAMN51253306			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_dloop_R2.fq.gz	003_Win_4_100m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_DL	003_Win_4_100m_01_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGCGGAAT	ATAGCGGAAT			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACTCTGCGC	TACTCTGCGC			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	150433	150433	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	430041	430041	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	84	84			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_032	GEM_R_dLoop3_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_dloop_R1.fq.gz	003_Win_4_100m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253306	SAMN51253306			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_dloop_R2.fq.gz	003_Win_4_100m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_DL	003_Win_4_100m_01_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGCGGAAT	ATAGCGGAAT			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACTCTGCGC	TACTCTGCGC			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	150433	150433	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	430041	430041	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	84	84			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_032	GEM_R_dLoop3_032			
CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:003_Win_4_100m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R1.fq.gz	003_Win_4_100m_02-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253310	SAMN51253310			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R2.fq.gz	003_Win_4_100m_02-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_DL	003_Win_4_100m_02-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGTTATTCTA	CGTTATTCTA			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACCTTATGG	AACCTTATGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	65010	65010	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	368072	368072	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	29920	29920			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_142	GEM_R_dLoop2_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R1.fq.gz	003_Win_4_100m_02-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253310	SAMN51253310			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R2.fq.gz	003_Win_4_100m_02-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_DL	003_Win_4_100m_02-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGTTATTCTA	CGTTATTCTA			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACCTTATGG	AACCTTATGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	65010	65010	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	368072	368072	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	29920	29920			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_142	GEM_R_dLoop2_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_33ca74ce7986b7c366cea509ae1e7bc8a8aebb11	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R1.fq.gz	003_Win_4_100m_02-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253310	SAMN51253310			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_dloop_R2.fq.gz	003_Win_4_100m_02-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-2_DL	003_Win_4_100m_02-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGTTATTCTA	CGTTATTCTA			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACCTTATGG	AACCTTATGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	65010	65010	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	368072	368072	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	29920	29920			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_142	GEM_R_dLoop2_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	CalCOFI_Intercal:DL:003_Win_4_100m_02-2_DL_occ_88e8dbb63d458323da39d429020b2fa2cad56737	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R1.fq.gz	003_Win_4_100m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253311	SAMN51253311			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R2.fq.gz	003_Win_4_100m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_DL	003_Win_4_100m_02-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACCGCCTCG	TACCGCCTCG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTTGCGAGG	GGTTGCGAGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	15999	15999	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	22722	22722	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2693	2693			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_142	GEM_R_dLoop3_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R1.fq.gz	003_Win_4_100m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253311	SAMN51253311			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R2.fq.gz	003_Win_4_100m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_DL	003_Win_4_100m_02-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACCGCCTCG	TACCGCCTCG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTTGCGAGG	GGTTGCGAGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	15999	15999	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	22722	22722	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2693	2693			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_142	GEM_R_dLoop3_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_47780b0475d5780cf2c0692ec13bba18c9c3caf0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R1.fq.gz	003_Win_4_100m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253311	SAMN51253311			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_dloop_R2.fq.gz	003_Win_4_100m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02-3_DL	003_Win_4_100m_02-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACCGCCTCG	TACCGCCTCG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTTGCGAGG	GGTTGCGAGG			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	15999	15999	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	22722	22722	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2693	2693			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_142	GEM_R_dLoop3_142			
CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	CalCOFI_Intercal:DL:003_Win_4_100m_02-3_DL_occ_c49afc26a1153be8e21b151ac6adcc4fe31d8231	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-2_dloop_R1.fq.gz	003_Win_4_100m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253313	SAMN51253313			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-2_dloop_R2.fq.gz	003_Win_4_100m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-2_DL	003_Win_4_100m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.17	6.17	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAAGTGGAC	ACAAGTGGAC			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACATCGCGC	AACATCGCGC			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	59709	59709	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	324243	324243	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	31270	31270			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_009	Rock3_dLoop2_009			
CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-2_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R1.fq.gz	003_Win_4_100m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253314	SAMN51253314			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R2.fq.gz	003_Win_4_100m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_DL	003_Win_4_100m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.17	6.17	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGCTCCGTT	CCGCTCCGTT			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTCGCCGTA	CTTCGCCGTA			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	159747	159747	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	450714	450714	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	11836	11836			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_009	Rock3_dLoop3_009			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R1.fq.gz	003_Win_4_100m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253314	SAMN51253314			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R2.fq.gz	003_Win_4_100m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_DL	003_Win_4_100m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.17	6.17	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGCTCCGTT	CCGCTCCGTT			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTCGCCGTA	CTTCGCCGTA			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	159747	159747	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	450714	450714	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	11836	11836			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_009	Rock3_dLoop3_009			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R1.fq.gz	003_Win_4_100m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253314	SAMN51253314			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_dloop_R2.fq.gz	003_Win_4_100m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03-3_DL	003_Win_4_100m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.17	6.17	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGCTCCGTT	CCGCTCCGTT			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTCGCCGTA	CTTCGCCGTA			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	159747	159747	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	450714	450714	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	11836	11836			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_009	Rock3_dLoop3_009			
CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	CalCOFI_Intercal:DL:003_Win_4_100m_03-3_DL_occ_77f3d8a28b03811e67855125dc204cb1eae92242	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03_dloop_R1.fq.gz	003_Win_4_100m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253312	SAMN51253312			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03_dloop_R2.fq.gz	003_Win_4_100m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_03_DL	003_Win_4_100m_03_DL			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.17	6.17	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACGGTCCAAC	ACGGTCCAAC			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGATGTAAGA	TGATGTAAGA			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	67443	67443	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	156679	156679	reads		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	17906	17906			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_009	Rock3_dLoop1_009			
CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_4_100m_03_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01-2_dloop_R1.fq.gz	003_Win_6_Chlamax_38m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253317	SAMN51253317			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01-2_dloop_R2.fq.gz	003_Win_6_Chlamax_38m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m	003_Win_6_Chlamax_38m			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01-2_DL	003_Win_6_Chlamax_38m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.846405229	1.846405229	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.135	0.135	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89299	5.89299	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.682	10.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.358	0.358	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.043	1.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	9.412	9.412	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.3	1.3	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGATCTGTGA	CGATCTGTGA			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGTCTTCCTA	AGTCTTCCTA			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.7157	13.7157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	38	38	meters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	92423	92423	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4875	33.4875	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	911069	911069	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	15299	15299			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_031	GEM_R_dLoop2_031			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_dloop_R1.fq.gz	003_Win_6_Chlamax_38m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253315	SAMN51253315			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_dloop_R2.fq.gz	003_Win_6_Chlamax_38m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m	003_Win_6_Chlamax_38m			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_DL	003_Win_6_Chlamax_38m_01_DL			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.846405229	1.846405229	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.135	0.135	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89299	5.89299	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.682	10.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.358	0.358	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.043	1.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	9.412	9.412	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.3	1.3	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTTCGGAGTT	GTTCGGAGTT			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGAAGGCG	TCAGAAGGCG			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.7157	13.7157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	38	38	meters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	40107	40107	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4875	33.4875	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	447803	447803	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2304	2304			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_031	GEM_R_dLoop3_031			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_dloop_R1.fq.gz	003_Win_6_Chlamax_38m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253315	SAMN51253315			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_dloop_R2.fq.gz	003_Win_6_Chlamax_38m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m	003_Win_6_Chlamax_38m			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01_DL	003_Win_6_Chlamax_38m_01_DL			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.846405229	1.846405229	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.135	0.135	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89299	5.89299	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.682	10.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.358	0.358	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.043	1.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	9.412	9.412	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.3	1.3	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTTCGGAGTT	GTTCGGAGTT			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGAAGGCG	TCAGAAGGCG			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.7157	13.7157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	38	38	meters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	40107	40107	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4875	33.4875	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	447803	447803	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2304	2304			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_031	GEM_R_dLoop3_031			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_01_DL_occ_bc44e42f31c46933cd9e73614cc4d891a6cec22b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_03-2_dloop_R1.fq.gz	003_Win_6_Chlamax_38m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253322	SAMN51253322			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_03-2_dloop_R2.fq.gz	003_Win_6_Chlamax_38m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m	003_Win_6_Chlamax_38m			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_03-2_DL	003_Win_6_Chlamax_38m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.846405229	1.846405229	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.135	0.135	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.89299	5.89299	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.682	10.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.358	0.358	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.043	1.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	9.412	9.412	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	30.27	30.27	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TATGCCTTAC	TATGCCTTAC			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TAATGTGTCT	TAATGTGTCT			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.7157	13.7157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	38	38	meters		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	272	272	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4875	33.4875	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	6571	6571	reads		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	003_Win_6_Chlamax_38m_01 | 003_Win_6_Chlamax_38m_02 | 003_Win_6_Chlamax_38m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	189	189			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_008	Rock3_dLoop2_008			
CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_6_Chlamax_38m_03-2_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R1.fq.gz	003_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253325	SAMN51253325			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R2.fq.gz	003_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_DL	003_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCAGGCA	CCACCAGGCA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTCCATAAG	ATTCCATAAG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	130279	130279	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	802316	802316	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58834	58834			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_030	GEM_R_dLoop1_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_0ed9fd00e9398f775e4cd29b4a7adfd9635130d0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R1.fq.gz	003_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253325	SAMN51253325			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R2.fq.gz	003_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_DL	003_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCAGGCA	CCACCAGGCA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTCCATAAG	ATTCCATAAG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	130279	130279	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	802316	802316	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58834	58834			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_030	GEM_R_dLoop1_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R1.fq.gz	003_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253325	SAMN51253325			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R2.fq.gz	003_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_DL	003_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCAGGCA	CCACCAGGCA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTCCATAAG	ATTCCATAAG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	130279	130279	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	802316	802316	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58834	58834			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_030	GEM_R_dLoop1_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R1.fq.gz	003_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253325	SAMN51253325			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_dloop_R2.fq.gz	003_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-1_DL	003_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCAGGCA	CCACCAGGCA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTCCATAAG	ATTCCATAAG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	130279	130279	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	802316	802316	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	58834	58834			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_030	GEM_R_dLoop1_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_dloop_R1.fq.gz	003_Win_8_10m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253326	SAMN51253326			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_dloop_R2.fq.gz	003_Win_8_10m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_DL	003_Win_8_10m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTCCTAGGA	CTTCCTAGGA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACTCTAGG	ATACTCTAGG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	122793	122793	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	820218	820218	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	41430	41430			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_030	GEM_R_dLoop2_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_dloop_R1.fq.gz	003_Win_8_10m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253326	SAMN51253326			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_dloop_R2.fq.gz	003_Win_8_10m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01-2_DL	003_Win_8_10m_01-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTCCTAGGA	CTTCCTAGGA			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACTCTAGG	ATACTCTAGG			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	122793	122793	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	820218	820218	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	41430	41430			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_030	GEM_R_dLoop2_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_dloop_R1.fq.gz	003_Win_8_10m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253324	SAMN51253324			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_dloop_R2.fq.gz	003_Win_8_10m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_DL	003_Win_8_10m_01_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACGCTGAA	CCACGCTGAA			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGAGGAATA	CTGAGGAATA			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	95995	95995	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	711757	711757	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8458	8458			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_030	GEM_R_dLoop3_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_dloop_R1.fq.gz	003_Win_8_10m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253324	SAMN51253324			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_dloop_R2.fq.gz	003_Win_8_10m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01_DL	003_Win_8_10m_01_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.69	0.69	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACGCTGAA	CCACGCTGAA			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGAGGAATA	CTGAGGAATA			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	95995	95995	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	711757	711757	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8458	8458			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_030	GEM_R_dLoop3_030			
CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_01_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02-3_dloop_R1.fq.gz	003_Win_8_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253328	SAMN51253328			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02-3_dloop_R2.fq.gz	003_Win_8_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02-3_DL	003_Win_8_10m_02-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGCAAGATAA	TGCAAGATAA			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGTCCTCAA	CCGTCCTCAA			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	75890	75890	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	564777	564777	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	60315	60315			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_133	GEM_R_dLoop3_133			
CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R1.fq.gz	003_Win_8_10m_02_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253327	SAMN51253327			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R2.fq.gz	003_Win_8_10m_02_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_DL	003_Win_8_10m_02_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGGCCTCTGT	TGGCCTCTGT			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCTGTCTCA	CGCTGTCTCA			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	511877	511877	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	1045057	1045057	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	104858	104858			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_133	GEM_R_dLoop1_133			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R1.fq.gz	003_Win_8_10m_02_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253327	SAMN51253327			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R2.fq.gz	003_Win_8_10m_02_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_DL	003_Win_8_10m_02_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGGCCTCTGT	TGGCCTCTGT			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCTGTCTCA	CGCTGTCTCA			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	511877	511877	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	1045057	1045057	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	104858	104858			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_133	GEM_R_dLoop1_133			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_7147e11bd5aa0d51ead0a6b3fee1007f6188e8c4	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R1.fq.gz	003_Win_8_10m_02_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253327	SAMN51253327			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_dloop_R2.fq.gz	003_Win_8_10m_02_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_DL	003_Win_8_10m_02_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGGCCTCTGT	TGGCCTCTGT			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGCTGTCTCA	CGCTGTCTCA			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	511877	511877	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	1045057	1045057	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	104858	104858			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_133	GEM_R_dLoop1_133			
CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	CalCOFI_Intercal:DL:003_Win_8_10m_02_DL_occ_c385c8eebae4def3e8e22853af12912850c9bbe5	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_69bee9489495e1b79f386073741172373030a5c3	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R1.fq.gz	003_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253330	SAMN51253330			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_dloop_R2.fq.gz	003_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-2_DL	003_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGTTACAA	ACCGTTACAA			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCGCTATGAG	TCGCTATGAG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28167	28167	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	50338	50338	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	28021	28021			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_007	Rock3_dLoop2_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	CalCOFI_Intercal:DL:003_Win_8_10m_03-2_DL_occ_b70d61347e9318b5ba868dfbe38e703152d7bb66	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_0b0bb8cbab169ef3811126fbe74a6eabd39a5398	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_21a67901174248780c9d5c401bb122ae463b8cc4	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_4b36f86369052d703fe7303a757a34a1ff870037	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R1.fq.gz	003_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253331	SAMN51253331			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_dloop_R2.fq.gz	003_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03-3_DL	003_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGAGGTCC	ATTGAGGTCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATAATCCG	ATATAATCCG			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	170951	170951	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259287	259287	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	170325	170325			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_007	Rock3_dLoop3_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:003_Win_8_10m_03-3_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R1.fq.gz	003_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253329	SAMN51253329			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R2.fq.gz	003_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_DL	003_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCCTACAGC	TTCCTACAGC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGAATTCGCC	AGAATTCGCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	98960	98960	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	128506	128506	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	97145	97145			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_007	Rock3_dLoop1_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R1.fq.gz	003_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253329	SAMN51253329			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R2.fq.gz	003_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_DL	003_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCCTACAGC	TTCCTACAGC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGAATTCGCC	AGAATTCGCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	98960	98960	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	128506	128506	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	97145	97145			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_007	Rock3_dLoop1_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R1.fq.gz	003_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253329	SAMN51253329			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R2.fq.gz	003_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_DL	003_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCCTACAGC	TTCCTACAGC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGAATTCGCC	AGAATTCGCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	98960	98960	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	128506	128506	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	97145	97145			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_007	Rock3_dLoop1_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_4d0300862d4665eb8993aeda05149c7654614745	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R1.fq.gz	003_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253329	SAMN51253329			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R2.fq.gz	003_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_DL	003_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCCTACAGC	TTCCTACAGC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGAATTCGCC	AGAATTCGCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	98960	98960	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	128506	128506	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	97145	97145			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_007	Rock3_dLoop1_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_ade1a89d8d8a0f2f1e00b0dad0d653957a3f596b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R1.fq.gz	003_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253329	SAMN51253329			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_dloop_R2.fq.gz	003_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_03_DL	003_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	28.29	28.29	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCCTACAGC	TTCCTACAGC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGAATTCGCC	AGAATTCGCC			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	98960	98960	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	128506	128506	reads		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	97145	97145			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_007	Rock3_dLoop1_007			
CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	CalCOFI_Intercal:DL:003_Win_8_10m_03_DL_occ_b2d05fb88a181dc98696cb4e2b185381e337f153	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	1500	1500	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-1_dloop_R1.fq.gz	004_GEM_5_Chlamax_17m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253104	SAMN51253104			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-1_dloop_R2.fq.gz	004_GEM_5_Chlamax_17m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m	004_GEM_5_Chlamax_17m			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-1_DL	004_GEM_5_Chlamax_17m-1_DL			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.834914611	1.834914611	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.158	0.158	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.34291	5.34291	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.66	1.66	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.075	0.075	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.365	0.365	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.0	2.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.84	0.84	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P245D	P245D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTGCGGAAC	CCTGCGGAAC			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGCCTATGAT	AGCCTATGAT			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.6524	18.6524	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	94906	94906	reads		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5375	33.5375	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	388696	388696	reads		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	609	609			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_073	GEM_R_dLoop1_073			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-1_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-2_dloop_R1.fq.gz	004_GEM_5_Chlamax_17m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253105	SAMN51253105			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-2_dloop_R2.fq.gz	004_GEM_5_Chlamax_17m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m	004_GEM_5_Chlamax_17m			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_5_Chlamax_17m-2_DL	004_GEM_5_Chlamax_17m-2_DL			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.834914611	1.834914611	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.158	0.158	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.34291	5.34291	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.66	1.66	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.075	0.075	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.365	0.365	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.0	2.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.84	0.84	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P245D	P245D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAACGACCTA	CAACGACCTA			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATCGAGTCGC	ATCGAGTCGC			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.6524	18.6524	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	164325	164325	reads		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5375	33.5375	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	973945	973945	reads		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1421	1421			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_073	GEM_R_dLoop2_073			
CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	CalCOFI_Intercal:DL:004_GEM_5_Chlamax_17m-2_DL_occ_daf62a3870544e6135da0c1ef9dd9e014d60cd17	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_dloop_R1.fq.gz	004_GEM_7_10m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253107	SAMN51253107			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_dloop_R2.fq.gz	004_GEM_7_10m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	900	900	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m	004_GEM_7_10m			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_DL	004_GEM_7_10m-1_DL			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32278	5.32278	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.54	0.54	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P245D	P245D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACACAGGTGG	ACACAGGTGG			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAACGCTCA	ACAACGCTCA			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8495	18.8495	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	101849	101849	reads		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	481304	481304	reads		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5184	5184			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_072	GEM_R_dLoop1_072			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	225	225	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_dloop_R1.fq.gz	004_GEM_7_10m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253107	SAMN51253107			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_dloop_R2.fq.gz	004_GEM_7_10m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	900	900	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m	004_GEM_7_10m			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_GEM_7_10m-1_DL	004_GEM_7_10m-1_DL			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32278	5.32278	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.54	0.54	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P245D	P245D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACACAGGTGG	ACACAGGTGG			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAACGCTCA	ACAACGCTCA			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8495	18.8495	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	101849	101849	reads		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	481304	481304	reads		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5184	5184			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_072	GEM_R_dLoop1_072			
CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	CalCOFI_Intercal:DL:004_GEM_7_10m-1_DL_occ_e9ace2d2b903d0232ac8a8c81c0d0d2bd41d58ca	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	225	225	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R1.fq.gz	004_Win_2_80m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253333	SAMN51253333			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R2.fq.gz	004_Win_2_80m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_DL	004_Win_2_80m_01-1_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTAACTCGC	GGTAACTCGC			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCACCAACTT	TCACCAACTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	180584	180584	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	789765	789765	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5243	5243			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_035	GEM_R_dLoop1_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R1.fq.gz	004_Win_2_80m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253333	SAMN51253333			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R2.fq.gz	004_Win_2_80m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_DL	004_Win_2_80m_01-1_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTAACTCGC	GGTAACTCGC			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCACCAACTT	TCACCAACTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	180584	180584	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	789765	789765	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5243	5243			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_035	GEM_R_dLoop1_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R1.fq.gz	004_Win_2_80m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253333	SAMN51253333			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R2.fq.gz	004_Win_2_80m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_DL	004_Win_2_80m_01-1_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTAACTCGC	GGTAACTCGC			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCACCAACTT	TCACCAACTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	180584	180584	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	789765	789765	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5243	5243			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_035	GEM_R_dLoop1_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_4ed4f30038ae9e2478d7f854e20152883c041094	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R1.fq.gz	004_Win_2_80m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253333	SAMN51253333			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_dloop_R2.fq.gz	004_Win_2_80m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01-1_DL	004_Win_2_80m_01-1_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTAACTCGC	GGTAACTCGC			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCACCAACTT	TCACCAACTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	180584	180584	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	789765	789765	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5243	5243			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_035	GEM_R_dLoop1_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	CalCOFI_Intercal:DL:004_Win_2_80m_01-1_DL_occ_fb299256bb5da2cbde199345d7809f72eaadedae	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_dloop_R1.fq.gz	004_Win_2_80m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253332	SAMN51253332			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_dloop_R2.fq.gz	004_Win_2_80m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_DL	004_Win_2_80m_01_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGATGCGGTT	CGATGCGGTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCAAGCAGG	ACCAAGCAGG			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	89338	89338	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	230658	230658	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	297	297			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_035	GEM_R_dLoop3_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_dloop_R1.fq.gz	004_Win_2_80m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253332	SAMN51253332			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_dloop_R2.fq.gz	004_Win_2_80m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_DL	004_Win_2_80m_01_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGATGCGGTT	CGATGCGGTT			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCAAGCAGG	ACCAAGCAGG			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	89338	89338	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	230658	230658	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	297	297			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_035	GEM_R_dLoop3_035			
CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:004_Win_2_80m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_dloop_R1.fq.gz	004_Win_2_80m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253339	SAMN51253339			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_dloop_R2.fq.gz	004_Win_2_80m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_DL	004_Win_2_80m_03-2_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.44	11.44	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTCTACATG	CCTCTACATG			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGAGGTATC	AGGAGGTATC			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	758	758	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	8149	8149	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	442	442			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_012	Rock3_dLoop2_012			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_dloop_R1.fq.gz	004_Win_2_80m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253339	SAMN51253339			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_dloop_R2.fq.gz	004_Win_2_80m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-2_DL	004_Win_2_80m_03-2_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.44	11.44	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTCTACATG	CCTCTACATG			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGAGGTATC	AGGAGGTATC			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	758	758	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	8149	8149	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	442	442			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_012	Rock3_dLoop2_012			
CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-2_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R1.fq.gz	004_Win_2_80m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253340	SAMN51253340			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R2.fq.gz	004_Win_2_80m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_DL	004_Win_2_80m_03-3_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.44	11.44	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACCTAGCAC	CACCTAGCAC			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGTCCAGCA	ATGTCCAGCA			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	253177	253177	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	377505	377505	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	46546	46546			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_012	Rock3_dLoop3_012			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R1.fq.gz	004_Win_2_80m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253340	SAMN51253340			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R2.fq.gz	004_Win_2_80m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_DL	004_Win_2_80m_03-3_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.44	11.44	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACCTAGCAC	CACCTAGCAC			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGTCCAGCA	ATGTCCAGCA			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	253177	253177	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	377505	377505	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	46546	46546			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_012	Rock3_dLoop3_012			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_4c78a69dbb604932090a10b7edb0a89600493fa9	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R1.fq.gz	004_Win_2_80m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253340	SAMN51253340			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_dloop_R2.fq.gz	004_Win_2_80m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_03-3_DL	004_Win_2_80m_03-3_DL			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.44	11.44	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACCTAGCAC	CACCTAGCAC			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGTCCAGCA	ATGTCCAGCA			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	253177	253177	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	377505	377505	reads		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	46546	46546			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_012	Rock3_dLoop3_012			
CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	CalCOFI_Intercal:DL:004_Win_2_80m_03-3_DL_occ_e21f95c6a108ea09a950ef21e75b3b7972ee11e6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_dloop_R1.fq.gz	004_Win_4_Chlamax_17m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253348	SAMN51253348			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_dloop_R2.fq.gz	004_Win_4_Chlamax_17m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m	004_Win_4_Chlamax_17m			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_DL	004_Win_4_Chlamax_17m_03-2_DL			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.834914611	1.834914611	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.158	0.158	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.34295	5.34295	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.66	1.66	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.075	0.075	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.365	0.365	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.0	2.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	26.07	26.07	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGGAATTCC	TTGGAATTCC			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTATTGACGT	GTATTGACGT			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.6522	18.6522	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	9340	9340	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5375	33.5375	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	23250	23250	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5895	5895			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_011	Rock3_dLoop2_011			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_dloop_R1.fq.gz	004_Win_4_Chlamax_17m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253348	SAMN51253348			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_dloop_R2.fq.gz	004_Win_4_Chlamax_17m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m	004_Win_4_Chlamax_17m			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-2_DL	004_Win_4_Chlamax_17m_03-2_DL			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.834914611	1.834914611	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.158	0.158	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.34295	5.34295	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.66	1.66	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.075	0.075	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.365	0.365	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.0	2.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	26.07	26.07	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGGAATTCC	TTGGAATTCC			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTATTGACGT	GTATTGACGT			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.6522	18.6522	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	9340	9340	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5375	33.5375	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	23250	23250	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5895	5895			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_011	Rock3_dLoop2_011			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-3_dloop_R1.fq.gz	004_Win_4_Chlamax_17m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253349	SAMN51253349			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-3_dloop_R2.fq.gz	004_Win_4_Chlamax_17m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m	004_Win_4_Chlamax_17m			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_03-3_DL	004_Win_4_Chlamax_17m_03-3_DL			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.834914611	1.834914611	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.158	0.158	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.34295	5.34295	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.66	1.66	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.075	0.075	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.365	0.365	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.0	2.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	26.07	26.07	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TAGGTATGTT	TAGGTATGTT			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTGGTCTCG	CTTGGTCTCG			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.6522	18.6522	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	245081	245081	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5375	33.5375	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	307405	307405	reads		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	004_Win_4_Chlamax_17m_01 | 004_Win_4_Chlamax_17m_02 | 004_Win_4_Chlamax_17m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	15874	15874			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_011	Rock3_dLoop3_011			
CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	CalCOFI_Intercal:DL:004_Win_4_Chlamax_17m_03-3_DL_occ_e364d5aa74f41180f24e076ffe3817d3f2ac3e95	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R1.fq.gz	004_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253356	SAMN51253356			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R2.fq.gz	004_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m	004_Win_8_10m			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_DL	004_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32075	5.32075	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.82	23.82	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACTTGGT	GTAACTTGGT			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGTATGGCG	AGGTATGGCG			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8706	18.8706	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	223772	223772	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	315866	315866	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	215492	215492			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_010	Rock3_dLoop1_010			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_44c59a6dfc2da83604fa6e1afb8b8589704b9301	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R1.fq.gz	004_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253356	SAMN51253356			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R2.fq.gz	004_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m	004_Win_8_10m			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_DL	004_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32075	5.32075	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.82	23.82	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACTTGGT	GTAACTTGGT			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGTATGGCG	AGGTATGGCG			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8706	18.8706	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	223772	223772	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	315866	315866	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	215492	215492			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_010	Rock3_dLoop1_010			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_5d5406e81691aef0138c4e1106fd964e88ec6c62	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R1.fq.gz	004_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253356	SAMN51253356			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R2.fq.gz	004_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m	004_Win_8_10m			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_DL	004_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32075	5.32075	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.82	23.82	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACTTGGT	GTAACTTGGT			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGTATGGCG	AGGTATGGCG			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8706	18.8706	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	223772	223772	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	315866	315866	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	215492	215492			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_010	Rock3_dLoop1_010			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_ee7b4e8138a9d58b7536e7830054fd6799e67a87	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R1.fq.gz	004_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253356	SAMN51253356			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_dloop_R2.fq.gz	004_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m	004_Win_8_10m			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_03_DL	004_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.705882353	1.705882353	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.32075	5.32075	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.63	0.63	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.263	0.263	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.252	1.252	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.82	23.82	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACTTGGT	GTAACTTGGT			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGTATGGCG	AGGTATGGCG			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.8706	18.8706	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	223772	223772	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.568	33.568	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	315866	315866	reads		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	004_Win_8_10m_01 | 004_Win_8_10m_02 | 004_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	215492	215492			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_010	Rock3_dLoop1_010			
CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	CalCOFI_Intercal:DL:004_Win_8_10m_03_DL_occ_f31a8cd9501b8cf3e1840c86bdeb3d19167d9a5e	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_GEM_1_380m-2_dloop_R1.fq.gz	005_GEM_1_380m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253110	SAMN51253110			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_GEM_1_380m-2_dloop_R2.fq.gz	005_GEM_1_380m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_GEM_1_380m	005_GEM_1_380m			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_GEM_1_380m-2_DL	005_GEM_1_380m-2_DL			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	missing	missing	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.106	0.106	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.69284	6.69284	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4.639	4.639	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.111	0.111	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.636	0.636	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	4.033	4.033	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	missing: not provided	missing: not provided	nanograms per microlitre		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P245D	P245D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATCTGCTT	ATATCTGCTT			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGATTGTCTA	AGATTGTCTA			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:30:00	19:30:00			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	7.6466	7.6466	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:30-07:00	2022-10-14T19:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	380	380	meters		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	133	133	reads		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	34.2707	34.2707	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	1702	1702	reads		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	13	13			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		DNA concentration "too low" to be read by Qubit, ie less than 0.05 ng/uL	DNA concentration "too low" to be read by Qubit, ie less than 0.05 ng/uL			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_079	GEM_R_dLoop2_079			
CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_GEM_1_380m-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01-2_dloop_R1.fq.gz	005_Win_6_Chlamax_31m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253370	SAMN51253370			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01-2_dloop_R2.fq.gz	005_Win_6_Chlamax_31m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m	005_Win_6_Chlamax_31m			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01-2_DL	005_Win_6_Chlamax_31m_01-2_DL			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.803030303	1.803030303	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.154	0.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.60462	5.60462	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4.348	4.348	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.103	0.103	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.594	0.594	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	3.662	3.662	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.5	2.5	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTGACCACT	CCTGACCACT			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGCCAAGA	ATAGCCAAGA			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.2157	16.2157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	31	31	meters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	68378	68378	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4555	33.4555	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	673685	673685	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2	2			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_037	GEM_R_dLoop2_037			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_01-2_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_dloop_R1.fq.gz	005_Win_6_Chlamax_31m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253375	SAMN51253375			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_dloop_R2.fq.gz	005_Win_6_Chlamax_31m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m	005_Win_6_Chlamax_31m			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_DL	005_Win_6_Chlamax_31m_03-2_DL			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.803030303	1.803030303	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.154	0.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.60462	5.60462	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4.348	4.348	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.103	0.103	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.594	0.594	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	3.662	3.662	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	17.08	17.08	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTCCGTAAGC	GTCCGTAAGC			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AAGATACACG	AAGATACACG			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.2157	16.2157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	31	31	meters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	32831	32831	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4555	33.4555	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	180302	180302	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4690	4690			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_014	Rock3_dLoop2_014			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_dloop_R1.fq.gz	005_Win_6_Chlamax_31m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253375	SAMN51253375			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_dloop_R2.fq.gz	005_Win_6_Chlamax_31m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m	005_Win_6_Chlamax_31m			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_03-2_DL	005_Win_6_Chlamax_31m_03-2_DL			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.803030303	1.803030303	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.154	0.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.60462	5.60462	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4.348	4.348	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.103	0.103	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.594	0.594	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	3.662	3.662	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	17.08	17.08	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTCCGTAAGC	GTCCGTAAGC			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AAGATACACG	AAGATACACG			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.2157	16.2157	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	31	31	meters		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	32831	32831	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4555	33.4555	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	180302	180302	reads		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	005_Win_6_Chlamax_31m_01 | 005_Win_6_Chlamax_31m_02 | 005_Win_6_Chlamax_31m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4690	4690			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_014	Rock3_dLoop2_014			
CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	CalCOFI_Intercal:DL:005_Win_6_Chlamax_31m_03-2_DL_occ_ad6e18d69ebf199b4b13a963f7d6373697f6bfbf	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_04f9e631d30b2d16ff68b9089ecebf77b1056335	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0821ac8721c5206f0a255dd8a6f70624dba65ee0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_0fd0c447f5ff8eee6f4c041e950e5563c489d845	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_1cd924f06e2f91f265e1d726b9bba3ffe04c8e20	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_2fe816208477b355b0eb2e01cdf13ab271850d36	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_32e805f3c30a9060349c72875a1031c78e61ca83	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3387c32f35278b4d07593ffdfec29706923c41e3	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_37206a03bfd998fc51dc5523e9981d58483d3b91	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d003dac25b8ebaeff5d71f7f5e4a54174169723	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3d6d6c85d94d067eb830f070ad6416f439e6f686	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_3f6a4926e9bad6b8ae82b55b6e32f56628ee7cfd	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43807aa16556913156c667847dea88e3a97895eb	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_43af0e6fbedc07e15f92b60b2fd78e69a9947d42	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_4593c4540ac4010c024acfe25203d61dfff25bac	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_525e6accc27bac41b45f334a6574593990fc488c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_56a8a0b6c23eebd48d1366594b9d6f7ac12db139	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_57ef4a8ccec7867e8027c21f0227a31f60fce97e	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5c7f61436578395cdbf9f5ffd3d1a71cc86d1140	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5dde5fdab27ffd242dea614fde19d013bf7e998d	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_5e49479f2efce7fbd9e6271ecf868388aede2211	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6288c28b253eb4b5316a33631f2cf2aad0282e40	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_652b78143272ccb87c4d310a6f1fc5370e144c3e	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_6bfc2ecbb6a516af56a390308e2ea22500e4f7cf	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_78c802547d057a7a56c756356f52321331a5f922	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_8d7aee451e193758bd418836e1119c5af8269170	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_93916cf62756886561744cb6b985a578267ec244	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a6d1e3a84ac501b739c777864cb830b4461ed5e9	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a91223d97c079c88f4709652604a3d28cfa072d6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b2c04bd22f657ad0cc3a50b336b874c53d2e66f0	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_b8fceb8e2226c874aebd941cb3b5aaaf938f5149	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_c33fc996d6cb48a766e05afcb09ba4d3abd3656a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_da87703ca72da57e8e99f43d24f441d8436ac307	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e00e802303269942bc0fc002dc5c6af887f6fcee	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_e5f9f907af14ac8c40e2279c0bcebef2bac1a796	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_f4980212de0d6e8506b8ea8729594320fa5bded5	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_fc0d13e0c1fefbd455a832fc0e385854f0d5d609	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ff39f4e52c1f51f12a6f1ff8686fac0ea3a68bb8	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R1.fq.gz	005_Win_8_10m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253378	SAMN51253378			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_dloop_R2.fq.gz	005_Win_8_10m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01-1_DL	005_Win_8_10m_01-1_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCGGCCGTA	ACCGGCCGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AATATTGCCA	AATATTGCCA			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3853568	3853568	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	7372033	7372033	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	3227195	3227195			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_036	GEM_R_dLoop1_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	CalCOFI_Intercal:DL:005_Win_8_10m_01-1_DL_occ_ffa599687f0581ff6df7b123f1e0de6998909437	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01_dloop_R1.fq.gz	005_Win_8_10m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253377	SAMN51253377			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01_dloop_R2.fq.gz	005_Win_8_10m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01_DL	005_Win_8_10m_01_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.27	0.27	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCCGGACTAG	TCCGGACTAG			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTGTTCGTC	ATTGTTCGTC			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	59119	59119	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	308605	308605	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1560	1560			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_036	GEM_R_dLoop3_036			
CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:005_Win_8_10m_01_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-2_dloop_R1.fq.gz	005_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253384	SAMN51253384			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-2_dloop_R2.fq.gz	005_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-2_DL	005_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.06	8.06	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGAGCGTGTA	GGAGCGTGTA			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACTTACGGAT	ACTTACGGAT			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	580	580	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	9611	9611	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	322	322			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_013	Rock3_dLoop2_013			
CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-2_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-3_dloop_R1.fq.gz	005_Win_8_10m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253385	SAMN51253385			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-3_dloop_R2.fq.gz	005_Win_8_10m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m	005_Win_8_10m			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_03-3_DL	005_Win_8_10m_03-3_DL			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.686390533	1.686390533	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.025	0.025	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.23969	5.23969	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.305	0.305	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.022	0.022	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.214	0.214	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.042	1.042	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	8.06	8.06	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P334D	P334D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCAAGTATG	TTCAAGTATG			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGATACCATC	CGATACCATC			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	19.6775	19.6775	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	34441	34441	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5836	33.5836	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	151800	151800	reads		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	005_Win_8_10m_01 | 005_Win_8_10m_02 | 005_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	26350	26350			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_013	Rock3_dLoop3_013			
CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	CalCOFI_Intercal:DL:005_Win_8_10m_03-3_DL_occ_111287818fe0a1e5e29c9227af65a520bf7c63b1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_dloop_R1.fq.gz	006_Win_4_100m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253394	SAMN51253394			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_dloop_R2.fq.gz	006_Win_4_100m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m	006_Win_4_100m			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_DL	006_Win_4_100m_03-3_DL			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.498031496	1.498031496	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.065	0.065	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.33601	6.33601	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.603	18.603	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.449	1.449	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	16.968	16.968	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	5.68	5.68	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTAATAGCAC	TTAATAGCAC			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGACGTGGT	CAGACGTGGT			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:30:00	12:30:00			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.2817	10.2817	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T12:30-07:00	2022-10-15T12:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	92059	92059	reads		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6313	33.6313	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	270848	270848	reads		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_01 | 006_Win_4_100m_02 | 006_Win_4_100m_03	006_Win_4_100m_01 | 006_Win_4_100m_02 | 006_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	27	27			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; CalCOFI-specific station where CTD was cast		76.7 55.0	76.7 55.0			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_018	Rock3_dLoop3_018			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_0de8446317b0f3d02f2e5dccaa46d367413baf27	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_dloop_R1.fq.gz	006_Win_4_100m_03-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253394	SAMN51253394			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_dloop_R2.fq.gz	006_Win_4_100m_03-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m	006_Win_4_100m			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_03-3_DL	006_Win_4_100m_03-3_DL			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.498031496	1.498031496	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.065	0.065	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.33601	6.33601	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.603	18.603	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.449	1.449	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	16.968	16.968	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	5.68	5.68	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTAATAGCAC	TTAATAGCAC			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R3: RREAS Rep 3	dLoop R3: RREAS Rep 3			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGACGTGGT	CAGACGTGGT			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:30:00	12:30:00			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.2817	10.2817	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T12:30-07:00	2022-10-15T12:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	92059	92059	reads		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6313	33.6313	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	270848	270848	reads		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_4_100m_01 | 006_Win_4_100m_02 | 006_Win_4_100m_03	006_Win_4_100m_01 | 006_Win_4_100m_02 | 006_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	27	27			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; CalCOFI-specific station where CTD was cast		76.7 55.0	76.7 55.0			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop3_018	Rock3_dLoop3_018			
CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	CalCOFI_Intercal:DL:006_Win_4_100m_03-3_DL_occ_271acf66e58b16f8d7e8a4397d8aa89fb83c002b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R1.fq.gz	006_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253411	SAMN51253411			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R2.fq.gz	006_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m	006_Win_8_10m			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_DL	006_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.340707965	1.340707965	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.036	0.036	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.65051	5.65051	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.285	0.285	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	0.574	0.574	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	27.37	27.37	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGAAGGCG	TCAGAAGGCG			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TATGATGGCC	TATGATGGCC			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:30:00	12:30:00			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.8789	15.8789	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T12:30-07:00	2022-10-15T12:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	4786	4786	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2415	33.2415	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	49494	49494	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	480	480			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	User-defined field; CalCOFI-specific station where CTD was cast		76.7 55.0	76.7 55.0			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_016	Rock3_dLoop2_016			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_863e82ff1e487146f36267d88ac5c13a65473b1b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R1.fq.gz	006_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253411	SAMN51253411			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R2.fq.gz	006_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m	006_Win_8_10m			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_DL	006_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.340707965	1.340707965	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.036	0.036	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.65051	5.65051	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.285	0.285	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	0.574	0.574	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	27.37	27.37	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGAAGGCG	TCAGAAGGCG			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TATGATGGCC	TATGATGGCC			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:30:00	12:30:00			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.8789	15.8789	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T12:30-07:00	2022-10-15T12:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	4786	4786	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2415	33.2415	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	49494	49494	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	480	480			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	User-defined field; CalCOFI-specific station where CTD was cast		76.7 55.0	76.7 55.0			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_016	Rock3_dLoop2_016			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_bbbca52c2d47d06d7ea9ec85146b10a261b26701	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R1.fq.gz	006_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253411	SAMN51253411			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_dloop_R2.fq.gz	006_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m	006_Win_8_10m			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_03-2_DL	006_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.340707965	1.340707965	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.036	0.036	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.65051	5.65051	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.285	0.285	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	0.574	0.574	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	27.37	27.37	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAGAAGGCG	TCAGAAGGCG			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TATGATGGCC	TATGATGGCC			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:30:00	12:30:00			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.8789	15.8789	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T12:30-07:00	2022-10-15T12:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.87891833	34.87891833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	4786	4786	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2154567	-121.2154567			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2415	33.2415	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	49494	49494	reads		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	006_Win_8_10m_01 | 006_Win_8_10m_02 | 006_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	480	480			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	User-defined field; CalCOFI-specific station where CTD was cast		76.7 55.0	76.7 55.0			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_016	Rock3_dLoop2_016			
CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	CalCOFI_Intercal:DL:006_Win_8_10m_03-2_DL_occ_c45ceb04a608ca4ccc57d05ff22cd47e7e080700	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01-1_dloop_R1.fq.gz	007_Win_6_41m_01-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253423	SAMN51253423			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01-1_dloop_R2.fq.gz	007_Win_6_41m_01-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m	007_Win_6_41m			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01-1_DL	007_Win_6_41m_01-1_DL			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.395480226	1.395480226	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.043	0.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.97611	5.97611	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.577	10.577	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.129	0.129	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.927	0.927	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	7.058	7.058	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.3	2.3	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P255D	P255D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCATCTCGCC	CCATCTCGCC			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACCATAGAA	AACCATAGAA			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.119	13.119	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	41	41	meters		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	35371	35371	reads		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2926	33.2926	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	565502	565502	reads		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01 | 007_Win_6_41m_02 | 007_Win_6_41m_03	007_Win_6_41m_01 | 007_Win_6_41m_02 | 007_Win_6_41m_03	decimal degrees		
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	174	174			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_043	GEM_R_dLoop1_043			
CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_6_41m_01-1_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01-2_dloop_R1.fq.gz	007_Win_8_10m_01-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253433	SAMN51253433			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01-2_dloop_R2.fq.gz	007_Win_8_10m_01-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m	007_Win_8_10m			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01-2_DL	007_Win_8_10m_01-2_DL			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.650485437	1.650485437	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.119	0.119	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.55297	5.55297	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.121	1.121	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.096	0.096	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.328	0.328	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.902	1.902	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	5.9	5.9	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P255D	P255D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTACCGATTA	GTACCGATTA			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACGTTAGTT	AACGTTAGTT			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.7458	16.7458	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	66478	66478	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2684	33.2684	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	833069	833069	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	725	725			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_042	GEM_R_dLoop2_042			
CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	CalCOFI_Intercal:DL:007_Win_8_10m_01-2_DL_occ_8c054e27f649ed43e8d75e8eaffbe48b37619287	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_dloop_R1.fq.gz	007_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253438	SAMN51253438			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_dloop_R2.fq.gz	007_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m	007_Win_8_10m			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_DL	007_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.650485437	1.650485437	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.119	0.119	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.55297	5.55297	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.121	1.121	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.096	0.096	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.328	0.328	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.902	1.902	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	18.16	18.16	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCATAGATTG	TCATAGATTG			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACCTTAATC	CACCTTAATC			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.7458	16.7458	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	6740	6740	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2684	33.2684	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	144153	144153	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1863	1863			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_019	Rock3_dLoop2_019			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_22905d1ea6587bd3907dc7d1ddf8bcf82e62aee6	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_dloop_R1.fq.gz	007_Win_8_10m_03-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253438	SAMN51253438			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_dloop_R2.fq.gz	007_Win_8_10m_03-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m	007_Win_8_10m			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03-2_DL	007_Win_8_10m_03-2_DL			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.650485437	1.650485437	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.119	0.119	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.55297	5.55297	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.121	1.121	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.096	0.096	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.328	0.328	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.902	1.902	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	18.16	18.16	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCATAGATTG	TCATAGATTG			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R2: RREAS Rep 3	dLoop R2: RREAS Rep 3			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACCTTAATC	CACCTTAATC			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.7458	16.7458	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	6740	6740	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2684	33.2684	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	144153	144153	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1863	1863			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop2_019	Rock3_dLoop2_019			
CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	CalCOFI_Intercal:DL:007_Win_8_10m_03-2_DL_occ_fcfbfca2b5fc280881fe9b75fe77a721e902fc94	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03_dloop_R1.fq.gz	007_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253437	SAMN51253437			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03_dloop_R2.fq.gz	007_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m	007_Win_8_10m			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_03_DL	007_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.650485437	1.650485437	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.119	0.119	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.55297	5.55297	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.121	1.121	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.096	0.096	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.328	0.328	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.902	1.902	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	18.16	18.16	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P333D	P333D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCACGAGAC	TTCACGAGAC			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACAATCT	GTAACAATCT			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.7458	16.7458	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	28988	28988	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2684	33.2684	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	154848	154848	reads		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	007_Win_8_10m_01 | 007_Win_8_10m_02 | 007_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	17107	17107			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_019	Rock3_dLoop1_019			
CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	CalCOFI_Intercal:DL:007_Win_8_10m_03_DL_occ_07e04b9b8a40a7030602553a8e18b9e0acc40477	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R1.fq.gz	009_Win_8_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253463	SAMN51253463			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R2.fq.gz	009_Win_8_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m	009_Win_8_10m			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_DL	009_Win_8_10m_02-3_DL			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.685393258	1.685393258	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59009	5.59009	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.118	0.118	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.01	0.01	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.304	0.304	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.166	1.166	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.7	23.7	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P264D	P264D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGTTATATC	CTGTTATATC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGCTATAC	ACAGCTATAC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:03:00	12:03:00			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.5449	16.5449	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T12:03-07:00	2022-10-16T12:03-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	194190	194190	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	32.8099	32.8099	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	523290	523290	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	61493	61493			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	User-defined field; CalCOFI-specific station where CTD was cast		73.3 60.0	73.3 60.0			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_143	GEM_R_dLoop3_143			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_126e4d78b40d50ae5591cec471e481ba15cacbae	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R1.fq.gz	009_Win_8_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253463	SAMN51253463			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R2.fq.gz	009_Win_8_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m	009_Win_8_10m			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_DL	009_Win_8_10m_02-3_DL			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.685393258	1.685393258	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59009	5.59009	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.118	0.118	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.01	0.01	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.304	0.304	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.166	1.166	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.7	23.7	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P264D	P264D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGTTATATC	CTGTTATATC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGCTATAC	ACAGCTATAC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:03:00	12:03:00			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.5449	16.5449	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T12:03-07:00	2022-10-16T12:03-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	194190	194190	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	32.8099	32.8099	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	523290	523290	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	61493	61493			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	User-defined field; CalCOFI-specific station where CTD was cast		73.3 60.0	73.3 60.0			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_143	GEM_R_dLoop3_143			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_34f3a329273654e13a5c29c90b06725c72c2dcbc	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R1.fq.gz	009_Win_8_10m_02-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253463	SAMN51253463			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_dloop_R2.fq.gz	009_Win_8_10m_02-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m	009_Win_8_10m			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_02-3_DL	009_Win_8_10m_02-3_DL			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.685393258	1.685393258	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.027	0.027	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59009	5.59009	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.118	0.118	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.01	0.01	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.304	0.304	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.166	1.166	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	23.7	23.7	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P264D	P264D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGTTATATC	CTGTTATATC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACAGCTATAC	ACAGCTATAC			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:03:00	12:03:00			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.5449	16.5449	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T12:03-07:00	2022-10-16T12:03-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	194190	194190	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	32.8099	32.8099	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	523290	523290	reads		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	009_Win_8_10m_01 | 009_Win_8_10m_02 | 009_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	61493	61493			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	User-defined field; CalCOFI-specific station where CTD was cast		73.3 60.0	73.3 60.0			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_143	GEM_R_dLoop3_143			
CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	CalCOFI_Intercal:DL:009_Win_8_10m_02-3_DL_occ_66e457a24665006656e5ae3b530f1b29387050ac	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	010_Win_6_Chlamax_17m_03_dloop_R1.fq.gz	010_Win_6_Chlamax_17m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253482	SAMN51253482			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	010_Win_6_Chlamax_17m_03_dloop_R2.fq.gz	010_Win_6_Chlamax_17m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	010_Win_6_Chlamax_17m	010_Win_6_Chlamax_17m			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	010_Win_6_Chlamax_17m_03_DL	010_Win_6_Chlamax_17m_03_DL			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.734513274	1.734513274	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.513	0.513	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.77649	5.77649	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2.781	2.781	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.145	0.145	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.396	0.396	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	8.039	8.039	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	14.46	14.46	nanograms per microlitre		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P332D	P332D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TGTGATAACT	TGTGATAACT			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGGCGCCTG	ATGGCGCCTG			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:40:00	15:40:00			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	14.8322	14.8322	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T15:40-07:00	2022-10-16T15:40-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.48039167	35.48039167			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.48039167	35.48039167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.61482	-121.61482	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	17	17	meters		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	18949	18949	reads		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.61482	-121.61482			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.0675	33.0675	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	64978	64978	reads		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	010_Win_6_Chlamax_17m_01 | 010_Win_6_Chlamax_17m_02 | 010_Win_6_Chlamax_17m_03	010_Win_6_Chlamax_17m_01 | 010_Win_6_Chlamax_17m_02 | 010_Win_6_Chlamax_17m_03	decimal degrees		
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2	2			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		73.3 55.0	73.3 55.0			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_026	Rock3_dLoop1_026			
CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:010_Win_6_Chlamax_17m_03_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	012_GEM_1_60m-2_dloop_R1.fq.gz	012_GEM_1_60m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253195	SAMN51253195			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	012_GEM_1_60m-2_dloop_R2.fq.gz	012_GEM_1_60m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	012_GEM_1_60m	012_GEM_1_60m			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	012_GEM_1_60m-2_DL	012_GEM_1_60m-2_DL			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.672009864	1.672009864	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.618	0.618	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.03515	6.03515	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8.924	8.924	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.295	0.295	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.975	0.975	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	8.401	8.401	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.7	0.7	nanograms per microlitre		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P243D	P243D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GAAGCGGCAC	GAAGCGGCAC			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGTAGAGCCG	AGTAGAGCCG			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:48:00	20:48:00			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	12.588	12.588	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T20:48-07:00	2022-10-16T20:48-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	60	60	meters		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	40186	40186	reads		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4523	33.4523	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	699143	699143	reads		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	502	502			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	User-defined field; CalCOFI-specific station where CTD was cast		73.3 50.0	73.3 50.0			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_111	GEM_R_dLoop2_111			
CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	CalCOFI_Intercal:DL:012_GEM_1_60m-2_DL_occ_373789d31d2783152b4b85cba6019642b70fd3f1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	014_GEM_2_170m-1_dloop_R1.fq.gz	014_GEM_2_170m-1_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253214	SAMN51253214			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	014_GEM_2_170m-1_dloop_R2.fq.gz	014_GEM_2_170m-1_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	014_GEM_2_170m	014_GEM_2_170m			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	014_GEM_2_170m-1_DL	014_GEM_2_170m-1_DL			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.125	1.125	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.004	0.004	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.56519	6.56519	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	25.7	25.7	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.018	0.018	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.804	1.804	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	28.966	28.966	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.12	0.12	nanograms per microlitre		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P253D	P253D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CGGTTACGGC	CGGTTACGGC			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTATAGTCTT	CTATAGTCTT			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:20:00	15:20:00			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	8.6161	8.6161	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T15:20-07:00	2022-10-17T15:20-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.7642	35.7642			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.7642	35.7642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.6210783	-122.6210783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	170	170	meters		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	243625	243625	reads		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.6210783	-122.6210783			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.8566	33.8566	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	785043	785043	reads		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2	2			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; CalCOFI-specific station where CTD was cast		70.0 63.6	70.0 63.6			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_016	GEM_R_dLoop1_016			
CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	CalCOFI_Intercal:DL:014_GEM_2_170m-1_DL_occ_1a27556f48219d29f7c1a6fd5fae460d1ae6502f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	014_Win_8_10m_03_dloop_R1.fq.gz	014_Win_8_10m_03_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253586	SAMN51253586			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	014_Win_8_10m_03_dloop_R2.fq.gz	014_Win_8_10m_03_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	014_Win_8_10m	014_Win_8_10m			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	014_Win_8_10m_03_DL	014_Win_8_10m_03_DL			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.716763006	1.716763006	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.015	0.015	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.4724	5.4724	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.192	0.192	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.013	0.013	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.306	0.306	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.353	1.353	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.43	6.43	nanograms per microlitre		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P331D	P331D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTTACCGCAC	CTTACCGCAC			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	dLoop R1: RREAS Rep 3	dLoop R1: RREAS Rep 3			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGGTCTTA	ATAGGTCTTA			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	17.7034	17.7034	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T15:30-07:00	2022-10-17T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.7642	35.7642			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.7642	35.7642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.6210783	-122.6210783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	415800	415800	reads		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.6210783	-122.6210783			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	32.6032	32.6032	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	810679	810679	reads		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	014_Win_8_10m_01 | 014_Win_8_10m_02 | 014_Win_8_10m_03	014_Win_8_10m_01 | 014_Win_8_10m_02 | 014_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	38429	38429			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	User-defined field; CalCOFI-specific station where CTD was cast		70.0 63.6	70.0 63.6			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	User-defined field; Unique sample name according to sequencing run manifest		Rock3_dLoop1_036	Rock3_dLoop1_036			
CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	CalCOFI_Intercal:DL:014_Win_8_10m_03_DL_occ_96eb153a390cbe93df22ca281cc79bbc33be9c9a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_dloop_R1.fq.gz	015_GEM_5_Chlamax_20m-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253236	SAMN51253236			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_dloop_R2.fq.gz	015_GEM_5_Chlamax_20m-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m	015_GEM_5_Chlamax_20m			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_DL	015_GEM_5_Chlamax_20m-3_DL			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.761627907	1.761627907	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.131	0.131	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.7326	5.7326	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5.876	5.876	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.254	0.254	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.626	0.626	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	4.344	4.344	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.7	1.7	nanograms per microlitre		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P242D	P242D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTGCAACCT	CCTGCAACCT			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGACTCTAC	CTGACTCTAC			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:55:00	20:55:00			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.1091	15.1091	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T20:55-07:00	2022-10-17T20:55-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	20	20	meters		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3253	3253	reads		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3508	33.3508	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	33865	33865	reads		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	207	207			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	User-defined field; CalCOFI-specific station where CTD was cast		68.1 55.9	68.1 55.9			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_119	GEM_R_dLoop3_119			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_0d79c4b2ef3f7949126f3cec838ef2f3578e3fb5	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_dloop_R1.fq.gz	015_GEM_5_Chlamax_20m-3_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253236	SAMN51253236			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_dloop_R2.fq.gz	015_GEM_5_Chlamax_20m-3_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m	015_GEM_5_Chlamax_20m			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	015_GEM_5_Chlamax_20m-3_DL	015_GEM_5_Chlamax_20m-3_DL			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.761627907	1.761627907	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.131	0.131	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.7326	5.7326	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5.876	5.876	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.254	0.254	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.626	0.626	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	4.344	4.344	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.7	1.7	nanograms per microlitre		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P242D	P242D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCTGCAACCT	CCTGCAACCT			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGACTCTAC	CTGACTCTAC			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:55:00	20:55:00			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.1091	15.1091	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T20:55-07:00	2022-10-17T20:55-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	20	20	meters		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	3253	3253	reads		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3508	33.3508	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	33865	33865	reads		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	207	207			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; CalCOFI-specific station where CTD was cast		68.1 55.9	68.1 55.9			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_119	GEM_R_dLoop3_119			
CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	CalCOFI_Intercal:DL:015_GEM_5_Chlamax_20m-3_DL_occ_b1298402abde352f6f1a2d8343c4ea01f58b140a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_4_100m_01_dloop_R1.fq.gz	015_Win_4_100m_01_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253589	SAMN51253589			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_4_100m_01_dloop_R2.fq.gz	015_Win_4_100m_01_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_4_100m	015_Win_4_100m			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_4_100m_01_DL	015_Win_4_100m_01_DL			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.2	1.2	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.038	0.038	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.36604	6.36604	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.289	18.289	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.018	0.018	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.408	1.408	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.048	17.048	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.6	1.6	nanograms per microlitre		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P253D	P253D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GTAACTTGGT	GTAACTTGGT			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGTATGGCG	AGGTATGGCG			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	21:00:00	21:00:00			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.0388	10.0388	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T21:00-07:00	2022-10-17T21:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	127950	127950	reads		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6434	33.6434	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	703003	703003	reads		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_4_100m_01 | 015_Win_4_100m_03	015_Win_4_100m_01 | 015_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	309	309			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	User-defined field; CalCOFI-specific station where CTD was cast		68.1 55.9	68.1 55.9			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop3_063	GEM_R_dLoop3_063			
CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	CalCOFI_Intercal:DL:015_Win_4_100m_01_DL_occ_38440b8c170b495de16b9da6a55819b38c179e44	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_dloop_R1.fq.gz	016_GEM_5_5m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253253	SAMN51253253			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_dloop_R2.fq.gz	016_GEM_5_5m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m	016_GEM_5_5m			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_DL	016_GEM_5_5m-2_DL			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.798742138	1.798742138	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.088	0.088	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.39283	5.39283	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.536	0.536	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.056	0.056	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.287	0.287	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.826	1.826	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.33	1.33	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P241D	P241D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTTGCGAGG	GGTTGCGAGG			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGCTCTATT	TTGCTCTATT			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221022	20221022			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:50:00	20:50:00			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.1638	18.1638	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-18T20:50-07:00	2022-10-18T20:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	5	5	meters		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	72981	72981	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5637	33.5637	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	420017	420017	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2630	2630			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; CalCOFI-specific station where CTD was cast		83.6 51.5	83.6 51.5			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_123	GEM_R_dLoop2_123			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_dloop_R1.fq.gz	016_GEM_5_5m-2_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253253	SAMN51253253			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_dloop_R2.fq.gz	016_GEM_5_5m-2_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m	016_GEM_5_5m			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m-2_DL	016_GEM_5_5m-2_DL			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.798742138	1.798742138	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.088	0.088	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.39283	5.39283	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.536	0.536	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.056	0.056	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.287	0.287	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.826	1.826	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.33	1.33	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P241D	P241D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGTTGCGAGG	GGTTGCGAGG			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGCTCTATT	TTGCTCTATT			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221022	20221022			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:50:00	20:50:00			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.1638	18.1638	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-18T20:50-07:00	2022-10-18T20:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	5	5	meters		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	72981	72981	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5637	33.5637	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	420017	420017	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2630	2630			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; CalCOFI-specific station where CTD was cast		83.6 51.5	83.6 51.5			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop2_123	GEM_R_dLoop2_123			
CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	CalCOFI_Intercal:DL:016_GEM_5_5m-2_DL_occ_a978c0c04dcb28d47c142f47c5694c55d876575f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_dloop_R1.fq.gz	016_GEM_5_5m_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253252	SAMN51253252			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_dloop_R2.fq.gz	016_GEM_5_5m_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m	016_GEM_5_5m			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_DL	016_GEM_5_5m_DL			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.798742138	1.798742138	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.088	0.088	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.39283	5.39283	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.536	0.536	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.056	0.056	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.287	0.287	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.826	1.826	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.33	1.33	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P241D	P241D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGCCGTGGA	AGGCCGTGGA			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACGCACGAG	AACGCACGAG			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221022	20221022			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:50:00	20:50:00			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.1638	18.1638	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-18T20:50-07:00	2022-10-18T20:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	5	5	meters		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	122531	122531	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5637	33.5637	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	286342	286342	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2995	2995			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	User-defined field; CalCOFI-specific station where CTD was cast		83.6 51.5	83.6 51.5			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_123	GEM_R_dLoop1_123			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_7f2a0eecedcbc972f50eb86e51d6f8e8183249f2	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_dloop_R1.fq.gz	016_GEM_5_5m_dloop_R1.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253252	SAMN51253252			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_dloop_R2.fq.gz	016_GEM_5_5m_dloop_R2.fq.gz			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	1000	1000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m	016_GEM_5_5m			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	016_GEM_5_5m_DL	016_GEM_5_5m_DL			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.798742138	1.798742138	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.088	0.088	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.39283	5.39283	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.536	0.536	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.056	0.056	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.287	0.287	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.826	1.826	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	1.33	1.33	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-16T12:00-07:00	2023-06-16T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P241D	P241D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGGCCGTGGA	AGGCCGTGGA			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit	MagMAX™ Microbiome Ultra Nucleic Acid Isolation kit			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	physical | chemical	physical | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACGCACGAG	AACGCACGAG			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221022	20221022			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:50:00	20:50:00			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.1638	18.1638	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-18T20:50-07:00	2022-10-18T20:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.81013333	33.81013333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	5	5	meters		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP - https://doi.org/10.25923/pf1n-c698	GEMCAP - https://doi.org/10.25923/pf1n-c698			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	122531	122531	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.1284783	-120.1284783			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5637	33.5637	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	286342	286342	reads		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable	decimal degrees		
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2995	2995			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; CalCOFI-specific station where CTD was cast		83.6 51.5	83.6 51.5			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_dLoop1_123	GEM_R_dLoop1_123			
CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	CalCOFI_Intercal:DL:016_GEM_5_5m_DL_occ_a1c3f332a8ee7fb5d6597767906bed2803e3be48	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	250	250	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_12S_R1.fq.gz	001_Win_12_Chlamax_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253746	SAMN51253746			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_12S_R2.fq.gz	001_Win_12_Chlamax_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax	001_Win_12_Chlamax			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01_MiFish	001_Win_12_Chlamax_01_MiFish			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.751552795	1.751552795	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59093	5.59093	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.105	0.105	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.013	0.013	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.277	0.277	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	0.956	0.956	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.3	2.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATATGCATGT	ATATGCATGT			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCAGGCACCA	CCAGGCACCA			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	13:50:00	13:50:00			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.4056	16.4056	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T13:50-07:00	2022-10-13T13:50-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.03382833	34.03382833			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.03382833	34.03382833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.0563317	-121.0563317	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	24	24	meters		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	4	4	reads		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.0563317	-121.0563317			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2419	33.2419	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	44313	44313	reads		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	001_Win_12_Chlamax_01 | 001_Win_12_Chlamax_02 | 001_Win_12_Chlamax_03	001_Win_12_Chlamax_01 | 001_Win_12_Chlamax_02 | 001_Win_12_Chlamax_03	decimal degrees		
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	4	4			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; CalCOFI-specific station where CTD was cast		80.7 59.9	80.7 59.9			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_025	GEM_R_MiFish_025			
CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:001_Win_12_Chlamax_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R1.fq.gz	002_Win_2_90m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253750	SAMN51253750			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R2.fq.gz	002_Win_2_90m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m	002_Win_2_90m			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_MiFish	002_Win_2_90m_01_MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.351485149	1.351485149	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2712	6.2712	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.216	20.216	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.052	0.052	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.667	1.667	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.101	19.101	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.99	0.99	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACATCACA	ATACATCACA			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7061	10.7061	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	90	90	meters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	177	177	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7244	33.7244	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	176456	176456	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01 | 002_Win_2_90m_03	002_Win_2_90m_01 | 002_Win_2_90m_03	decimal degrees		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	81	81			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_029	GEM_R_MiFish_029			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_55415cc63ff526829c699a4c5000d21af65bfa80	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R1.fq.gz	002_Win_2_90m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253750	SAMN51253750			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R2.fq.gz	002_Win_2_90m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m	002_Win_2_90m			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_MiFish	002_Win_2_90m_01_MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.351485149	1.351485149	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2712	6.2712	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.216	20.216	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.052	0.052	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.667	1.667	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.101	19.101	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.99	0.99	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACATCACA	ATACATCACA			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7061	10.7061	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	90	90	meters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	177	177	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7244	33.7244	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	176456	176456	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01 | 002_Win_2_90m_03	002_Win_2_90m_01 | 002_Win_2_90m_03	decimal degrees		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	81	81			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_029	GEM_R_MiFish_029			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_79f49ab754d9653fe86402a43caef074eefc7d02	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R1.fq.gz	002_Win_2_90m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253750	SAMN51253750			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R2.fq.gz	002_Win_2_90m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m	002_Win_2_90m			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_MiFish	002_Win_2_90m_01_MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.351485149	1.351485149	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2712	6.2712	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.216	20.216	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.052	0.052	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.667	1.667	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.101	19.101	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.99	0.99	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACATCACA	ATACATCACA			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7061	10.7061	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	90	90	meters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	177	177	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7244	33.7244	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	176456	176456	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01 | 002_Win_2_90m_03	002_Win_2_90m_01 | 002_Win_2_90m_03	decimal degrees		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	81	81			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_029	GEM_R_MiFish_029			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_aa59062aed8d764a52ead5c2293d4950002d5bca	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R1.fq.gz	002_Win_2_90m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253750	SAMN51253750			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_12S_R2.fq.gz	002_Win_2_90m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m	002_Win_2_90m			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01_MiFish	002_Win_2_90m_01_MiFish			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.351485149	1.351485149	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2712	6.2712	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.216	20.216	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.052	0.052	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.667	1.667	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.101	19.101	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	0.99	0.99	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P257D	P257D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACATCACA	ATACATCACA			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7061	10.7061	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	90	90	meters		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	177	177	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7244	33.7244	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	176456	176456	reads		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_2_90m_01 | 002_Win_2_90m_03	002_Win_2_90m_01 | 002_Win_2_90m_03	decimal degrees		
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	81	81			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_029	GEM_R_MiFish_029			
CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	CalCOFI_Intercal:MiFish:002_Win_2_90m_01_MiFish_occ_d8b913a4eb4fec5a28ae192fb1b00a0483364a2b	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_4_Chlamax_30m_02_12S_R1.fq.gz	002_Win_4_Chlamax_30m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253751	SAMN51253751			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_4_Chlamax_30m_02_12S_R2.fq.gz	002_Win_4_Chlamax_30m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_4_Chlamax_30m	002_Win_4_Chlamax_30m			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_4_Chlamax_30m_02_MiFish	002_Win_4_Chlamax_30m_02_MiFish			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.792853155	1.792853155	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.82681	5.82681	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	7.443	7.443	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.389	0.389	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.825	0.825	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	5.888	5.888	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	21.3	21.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P267D	P267D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CACTATCAAC	CACTATCAAC			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CATTAACTGA	CATTAACTGA			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221013	20221013			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	18:30:00	18:30:00			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	14.2768	14.2768	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-13T18:30-07:00	2022-10-13T18:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.14325333	34.14325333		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	30	30	meters		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	12	12	reads		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.5919967	-120.5919967			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4758	33.4758	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	2940	2940	reads		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	002_Win_4_Chlamax_30m_01 | 002_Win_4_Chlamax_30m_02 | 002_Win_4_Chlamax_30m_03	002_Win_4_Chlamax_30m_01 | 002_Win_4_Chlamax_30m_02 | 002_Win_4_Chlamax_30m_03	decimal degrees		
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	12	12			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; CalCOFI-specific station where CTD was cast		81.2 53.9	81.2 53.9			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_139	GEM_R_MiFish_139			
CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:002_Win_4_Chlamax_30m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_12S_R1.fq.gz	003_Win_4_100m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253754	SAMN51253754			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_12S_R2.fq.gz	003_Win_4_100m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01_MiFish	003_Win_4_100m_01_MiFish			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.4	2.4	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AGTACTCATG	AGTACTCATG			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACCACGACAT	ACCACGACAT			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	111	111	reads		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	244281	244281	reads		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	72	72			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_032	GEM_R_MiFish_032			
CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:003_Win_4_100m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02_12S_R1.fq.gz	003_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253755	SAMN51253755			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02_12S_R2.fq.gz	003_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m	003_Win_4_100m			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_02_MiFish	003_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.240963855	1.240963855	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.2881	6.2881	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.795	18.795	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.498	1.498	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.5	17.5	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.9	9.9	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTAAGTACGC	CTAAGTACGC			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TAGGTATGTT	TAGGTATGTT			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.5905	10.5905	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	535	535	reads		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7298	33.7298	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	76560	76560	reads		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	003_Win_4_100m_01 | 003_Win_4_100m_02 | 003_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	535	535			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_142	GEM_R_MiFish_142			
CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	CalCOFI_Intercal:MiFish:003_Win_4_100m_02_MiFish_occ_f253232c786b648f2640895d9b2797f8a28bbc62	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R1.fq.gz	003_Win_8_10m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253761	SAMN51253761			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R2.fq.gz	003_Win_8_10m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_MiFish	003_Win_8_10m_02_MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCGGAGT	CCACCGGAGT			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CATGGCAGGC	CATGGCAGGC			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	1330	1330	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	302243	302243	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1312	1312			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_133	GEM_R_MiFish_133			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R1.fq.gz	003_Win_8_10m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253761	SAMN51253761			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R2.fq.gz	003_Win_8_10m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_MiFish	003_Win_8_10m_02_MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCGGAGT	CCACCGGAGT			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CATGGCAGGC	CATGGCAGGC			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	1330	1330	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	302243	302243	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1312	1312			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_133	GEM_R_MiFish_133			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_e98769be776b04467b19da766ed0ab33c4bc81c7	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R1.fq.gz	003_Win_8_10m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253761	SAMN51253761			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_12S_R2.fq.gz	003_Win_8_10m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m	003_Win_8_10m			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_02_MiFish	003_Win_8_10m_02_MiFish			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.749152542	1.749152542	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.122	0.122	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.40948	5.40948	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.136	0.136	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.024	0.024	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.257	0.257	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.154	1.154	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	11.2	11.2	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCACCGGAGT	CCACCGGAGT			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CATGGCAGGC	CATGGCAGGC			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:00:00	12:00:00			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	18.005	18.005	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T12:00-07:00	2022-10-14T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.138455	34.138455		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	1330	1330	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.875925	-119.875925			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.5656	33.5656	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	302243	302243	reads		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	003_Win_8_10m_01 | 003_Win_8_10m_02 | 003_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1312	1312			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	User-defined field; CalCOFI-specific station where CTD was cast		82.7 46.1	82.7 46.1			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	User-defined field; Observational notes related to sample collection		pod of 100 common dolphins on station at the surface	pod of 100 common dolphins on station at the surface			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_133	GEM_R_MiFish_133			
CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	CalCOFI_Intercal:MiFish:003_Win_8_10m_02_MiFish_occ_f79238cc6edeeb1784e5f5929e9e4a3ba16330ff	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_12S_R1.fq.gz	004_Win_2_80m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253763	SAMN51253763			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_12S_R2.fq.gz	004_Win_2_80m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m	004_Win_2_80m			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01_MiFish	004_Win_2_80m_01_MiFish			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.467353952	1.467353952	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.22475	6.22475	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	19.062	19.062	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.098	0.098	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.397	1.397	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	17.465	17.465	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.1	3.1	nanograms per microlitre		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P256D	P256D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATTAACAAGG	ATTAACAAGG			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ACGAACAACA	ACGAACAACA			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:30:00	15:30:00			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	11.0704	11.0704	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T15:30-07:00	2022-10-14T15:30-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	34.05617833	34.05617833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	80	80	meters		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	147	147	reads		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4034367	-119.4034367			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.6583	33.6583	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	119139	119139	reads		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	004_Win_2_80m_01 | 004_Win_2_80m_02 | 004_Win_2_80m_03	decimal degrees		
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	9	9			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	User-defined field; CalCOFI-specific station where CTD was cast		84.0 41.7	84.0 41.7			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	User-defined field; Observational notes related to sample collection		common dolphins, humpbacks, maybe a bottlenose dolphin	common dolphins, humpbacks, maybe a bottlenose dolphin			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_035	GEM_R_MiFish_035			
CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	CalCOFI_Intercal:MiFish:004_Win_2_80m_01_MiFish_occ_eb429c0912ae24ec7a424cb37bae2ace3faa9b64	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_4_100m_02_12S_R1.fq.gz	005_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253768	SAMN51253768			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_4_100m_02_12S_R2.fq.gz	005_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_4_100m	005_Win_4_100m			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_4_100m_02_MiFish	005_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.211111111	1.211111111	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.26415	6.26415	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	22.364	22.364	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.004	0.004	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.806	1.806	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	21.469	21.469	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.8	3.8	nanograms per microlitre		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P266D	P266D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CCGAAGCGCT	CCGAAGCGCT			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGTTAGCA	ATAGTTAGCA			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221014	20221014			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	19:10:00	19:10:00			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7627	10.7627	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-14T19:10-07:00	2022-10-14T19:10-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	33.92825667	33.92825667		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	361	361	reads		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-119.4754	-119.4754			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.801	33.801	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	193505	193505	reads		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	005_Win_4_100m_01 | 005_Win_4_100m_02 | 005_Win_4_100m_03	005_Win_4_100m_01 | 005_Win_4_100m_02 | 005_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	361	361			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	User-defined field; CalCOFI-specific station where CTD was cast		84.5 43.5	84.5 43.5			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	User-defined field; Observational notes related to sample collection		common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD	common dolphins, subnose pipefish, needlefish, minke whale surfaced near CTD			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_138	GEM_R_MiFish_138			
CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	CalCOFI_Intercal:MiFish:005_Win_4_100m_02_MiFish_occ_41cc3aa208549e4084e1b7e58822a8551f07d1dc	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R1.fq.gz	007_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253780	SAMN51253780			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R2.fq.gz	007_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m	007_Win_4_100m			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_MiFish	007_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.183673469	1.183673469	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.014	0.014	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.27059	6.27059	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.629	20.629	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.682	1.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.997	19.997	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.3	9.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P265D	P265D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGCTCTATT	TTGCTCTATT			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAAGGTCAC	TCAAGGTCAC			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7253	10.7253	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	288	288	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7138	33.7138	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	196787	196787	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	232	232			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_140	GEM_R_MiFish_140			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_3be89c1551c375dd77213298b37229cdd8fce2f1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R1.fq.gz	007_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253780	SAMN51253780			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R2.fq.gz	007_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m	007_Win_4_100m			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_MiFish	007_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.183673469	1.183673469	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.014	0.014	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.27059	6.27059	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.629	20.629	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.682	1.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.997	19.997	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.3	9.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P265D	P265D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGCTCTATT	TTGCTCTATT			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAAGGTCAC	TCAAGGTCAC			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7253	10.7253	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	288	288	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7138	33.7138	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	196787	196787	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	232	232			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_140	GEM_R_MiFish_140			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R1.fq.gz	007_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253780	SAMN51253780			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_12S_R2.fq.gz	007_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m	007_Win_4_100m			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_02_MiFish	007_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.183673469	1.183673469	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.014	0.014	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.27059	6.27059	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	20.629	20.629	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.019	0.019	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.682	1.682	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	19.997	19.997	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	9.3	9.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P265D	P265D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTGCTCTATT	TTGCTCTATT			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCAAGGTCAC	TCAAGGTCAC			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.7253	10.7253	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	288	288	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.7138	33.7138	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	196787	196787	reads		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	007_Win_4_100m_01 | 007_Win_4_100m_02 | 007_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	232	232			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_140	GEM_R_MiFish_140			
CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	CalCOFI_Intercal:MiFish:007_Win_4_100m_02_MiFish_occ_d35e32477ff076dab13bc2d5d6550ce21e069b19	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01_12S_R1.fq.gz	007_Win_6_41m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253781	SAMN51253781			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01_12S_R2.fq.gz	007_Win_6_41m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m	007_Win_6_41m			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01_MiFish	007_Win_6_41m_01_MiFish			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.395480226	1.395480226	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.043	0.043	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.97611	5.97611	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	10.577	10.577	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.129	0.129	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.927	0.927	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	7.058	7.058	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.3	2.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P255D	P255D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TCCGCCAATT	TCCGCCAATT			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCACGGAG	CAGCACGGAG			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221015	20221015			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	15:45:00	15:45:00			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	13.119	13.119	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-15T15:45-07:00	2022-10-15T15:45-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.02221167	35.02221167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	41	41	meters		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	142	142	reads		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-120.9179683	-120.9179683			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.2926	33.2926	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	115239	115239	reads		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	007_Win_6_41m_01 | 007_Win_6_41m_02 | 007_Win_6_41m_03	007_Win_6_41m_01 | 007_Win_6_41m_02 | 007_Win_6_41m_03	decimal degrees		
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	16	16			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; CalCOFI-specific station where CTD was cast		76.7 51.0	76.7 51.0			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Observational notes related to sample collection		smell of sewage on station, smell in lab, and on all following stations for the trip	smell of sewage on station, smell in lab, and on all following stations for the trip			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_043	GEM_R_MiFish_043			
CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	CalCOFI_Intercal:MiFish:007_Win_6_41m_01_MiFish_occ_57f85271465bc271cfee71c8f628ab2e6d187d29	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_4_100m_02_12S_R1.fq.gz	009_Win_4_100m_02_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253786	SAMN51253786			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_4_100m_02_12S_R2.fq.gz	009_Win_4_100m_02_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_4_100m	009_Win_4_100m			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_4_100m_02_MiFish	009_Win_4_100m_02_MiFish			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.159090909	1.159090909	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.0	0.0	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.29825	6.29825	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18.09	18.09	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.016	0.016	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	1.348	1.348	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	14.523	14.523	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	3.7	3.7	nanograms per microlitre		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-07-07T12:00-07:00	2023-07-07T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P264D	P264D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTATACGCGA	TTATACGCGA			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 2	GEMCAP/RREAS Plate 2			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACAGACGGC	AACAGACGGC			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:03:00	12:03:00			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	10.6086	10.6086	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T12:03-07:00	2022-10-16T12:03-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.30843167	35.30843167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	100	100	meters		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	159	159	reads		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.9586333	-121.9586333			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4148	33.4148	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	60422	60422	reads		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	009_Win_4_100m_01 | 009_Win_4_100m_02 | 009_Win_4_100m_03	009_Win_4_100m_01 | 009_Win_4_100m_02 | 009_Win_4_100m_03	decimal degrees		
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	73	73			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; CalCOFI-specific station where CTD was cast		73.3 60.0	73.3 60.0			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_136	GEM_R_MiFish_136			
CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	CalCOFI_Intercal:MiFish:009_Win_4_100m_02_MiFish_occ_6df43c1c63c8e8d8f8e2f4c3b19e3e73f496274a	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01_12S_R1.fq.gz	012_Win_2_60m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253794	SAMN51253794			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01_12S_R2.fq.gz	012_Win_2_60m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m	012_Win_2_60m			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01_MiFish	012_Win_2_60m_01_MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.672009864	1.672009864	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.618	0.618	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.03515	6.03515	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8.924	8.924	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.295	0.295	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.975	0.975	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	8.401	8.401	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	4.5	4.5	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P254D	P254D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TTCGGTGTGA	TTCGGTGTGA			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATACTTGTTC	ATACTTGTTC			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:48:00	20:48:00			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	12.588	12.588	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T20:48-07:00	2022-10-16T20:48-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	60	60	meters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	67	67	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4523	33.4523	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	88698	88698	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	decimal degrees		
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	22	22			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; CalCOFI-specific station where CTD was cast		73.3 50.0	73.3 50.0			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_055	GEM_R_MiFish_055			
CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	CalCOFI_Intercal:MiFish:012_Win_2_60m_01_MiFish_occ_45be7f59b82296342b15ad610f1be96b22f10a1c	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_12S_R1.fq.gz	012_Win_2_60m_03_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253796	SAMN51253796			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_12S_R2.fq.gz	012_Win_2_60m_03_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m	012_Win_2_60m			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_MiFish	012_Win_2_60m_03_MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.672009864	1.672009864	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.618	0.618	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.03515	6.03515	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8.924	8.924	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.295	0.295	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.975	0.975	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	8.401	8.401	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	10.21	10.21	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P332D	P332D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGTTGGTCC	CTGTTGGTCC			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS Rep 3	RREAS Rep 3			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACGGTCTAT	AACGGTCTAT			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:48:00	20:48:00			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	12.588	12.588	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T20:48-07:00	2022-10-16T20:48-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	60	60	meters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	119664	119664	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4523	33.4523	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	167123	167123	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	decimal degrees		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	63	63			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	User-defined field; CalCOFI-specific station where CTD was cast		73.3 50.0	73.3 50.0			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	User-defined field; Observational notes related to sample processing		filter touched the metal table, potentially contaminated	filter touched the metal table, potentially contaminated			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	User-defined field; Unique sample name according to sequencing run manifest		Rock3_MiFish_032	Rock3_MiFish_032			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_9feec0ab68c155d96006b0a1d2ae534a7a9200d1	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_12S_R1.fq.gz	012_Win_2_60m_03_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253796	SAMN51253796			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_12S_R2.fq.gz	012_Win_2_60m_03_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m	012_Win_2_60m			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_03_MiFish	012_Win_2_60m_03_MiFish			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.672009864	1.672009864	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.618	0.618	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	6.03515	6.03515	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	8.924	8.924	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.295	0.295	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.975	0.975	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	8.401	8.401	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	10.21	10.21	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-09-13T12:00-07:00	2023-09-13T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P332D	P332D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTGTTGGTCC	CTGTTGGTCC			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS Rep 3	RREAS Rep 3			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	AACGGTCTAT	AACGGTCTAT			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:48:00	20:48:00			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	12.588	12.588	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	16.9	16.9	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T20:48-07:00	2022-10-16T20:48-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	60	60	meters		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	119664	119664	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.4523	33.4523	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	167123	167123	reads		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	012_Win_2_60m_01 | 012_Win_2_60m_02 | 012_Win_2_60m_03	decimal degrees		
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	63	63			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	User-defined field; CalCOFI-specific station where CTD was cast		73.3 50.0	73.3 50.0			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	User-defined field; Observational notes related to sample processing		filter touched the metal table, potentially contaminated	filter touched the metal table, potentially contaminated			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	User-defined field; Unique sample name according to sequencing run manifest		Rock3_MiFish_032	Rock3_MiFish_032			
CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	CalCOFI_Intercal:MiFish:012_Win_2_60m_03_MiFish_occ_ae5c640d48cd25e466531b244eeb8d01b7e58164	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_4_10m_01_12S_R1.fq.gz	012_Win_4_10m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253797	SAMN51253797			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_4_10m_01_12S_R2.fq.gz	012_Win_4_10m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_4_10m	012_Win_4_10m			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_4_10m_01_MiFish	012_Win_4_10m_01_MiFish			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.853769301	1.853769301	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.168	0.168	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.59104	5.59104	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2.991	2.991	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.171	0.171	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.414	0.414	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.679	2.679	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	6.6	6.6	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P254D	P254D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATAGCCAAGA	ATAGCCAAGA			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGTGGCACT	CAGTGGCACT			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221016	20221016			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	20:48:00	20:48:00			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.3621	16.3621	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-16T20:48-07:00	2022-10-16T20:48-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.63402167	35.63402167		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	2	2	reads		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-121.2880883	-121.2880883			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3785	33.3785	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	35955	35955	reads		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	012_Win_4_10m_01 | 012_Win_4_10m_02 | 012_Win_4_10m_03	012_Win_4_10m_01 | 012_Win_4_10m_02 | 012_Win_4_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	2	2			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	User-defined field; CalCOFI-specific station where CTD was cast		73.3 50.0	73.3 50.0			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_054	GEM_R_MiFish_054			
CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	CalCOFI_Intercal:MiFish:012_Win_4_10m_01_MiFish_occ_9ec8a8689e333971375e239accf121168d1ce1d8	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	013_Win_8_10m_01_12S_R1.fq.gz	013_Win_8_10m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253801	SAMN51253801			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	013_Win_8_10m_01_12S_R2.fq.gz	013_Win_8_10m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	013_Win_8_10m	013_Win_8_10m			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	013_Win_8_10m_01_MiFish	013_Win_8_10m_01_MiFish			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.740506329	1.740506329	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.017	0.017	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.487	5.487	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.018	0.018	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.047	0.047	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.309	0.309	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	1.058	1.058	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	4.7	4.7	nanograms per microlitre		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P253D	P253D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	ATGTAACGTT	ATGTAACGTT			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CAGCAATCGT	CAGCAATCGT			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	12:15:00	12:15:00			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	17.5784	17.5784	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T12:15-07:00	2022-10-17T12:15-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.65952833	35.65952833			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	35.65952833	35.65952833		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.83645	-122.83645	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	77	77	reads		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.83645	-122.83645			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	32.5729	32.5729	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	259897	259897	reads		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	013_Win_8_10m_01 | 013_Win_8_10m_03	013_Win_8_10m_01 | 013_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	29	29			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; CalCOFI-specific station where CTD was cast		70.0 66.7	70.0 66.7			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_056	GEM_R_MiFish_056			
CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:013_Win_8_10m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_6_Chlamax_20m_01_12S_R1.fq.gz	015_Win_6_Chlamax_20m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253810	SAMN51253810			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_6_Chlamax_20m_01_12S_R2.fq.gz	015_Win_6_Chlamax_20m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_6_Chlamax_20m	015_Win_6_Chlamax_20m			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_6_Chlamax_20m_01_MiFish	015_Win_6_Chlamax_20m_01_MiFish			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.761627907	1.761627907	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.131	0.131	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.7326	5.7326	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	5.876	5.876	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.254	0.254	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.626	0.626	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	4.344	4.344	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2.9	2.9	nanograms per microlitre		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P253D	P253D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TACGTGAAGG	TACGTGAAGG			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	CTAATAACCG	CTAATAACCG			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	21:00:00	21:00:00			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	15.1091	15.1091	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T21:00-07:00	2022-10-17T21:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	20	20	meters		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	18	18	reads		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3508	33.3508	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	155163	155163	reads		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_6_Chlamax_20m_01 | 015_Win_6_Chlamax_20m_02 | 015_Win_6_Chlamax_20m_03	015_Win_6_Chlamax_20m_01 | 015_Win_6_Chlamax_20m_02 | 015_Win_6_Chlamax_20m_03	decimal degrees		
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	18	18			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; CalCOFI-specific station where CTD was cast		68.1 55.9	68.1 55.9			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_064	GEM_R_MiFish_064			
CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	CalCOFI_Intercal:MiFish:015_Win_6_Chlamax_20m_01_MiFish_occ_6212822656ef24ef6502d0cb4683e56823d7a031	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A brief, concise identifier for assay with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'assay_name'.		DL | MiFish	DL | MiFish			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A brief, concise identifier for sequencing run with no spaces or special characters, ensuring machine readability. This ID will be used in file names as 'seq_run_id'.	https://github.com/FAIR-eDNA/FAIRe_checklist	UCSD_NovaSeq_20240403	UCSD_NovaSeq_20240403			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A file name of raw sequencing data. If paired end, fill filename2 for the reverse read	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_8_10m_01_12S_R1.fq.gz	015_Win_8_10m_01_12S_R1.fq.gz			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A link to a litreature reference, electronic resource or a standard operating procedure (SOP), that describes the material separation to recover the nucleic acid fraction from a sample. If a custom extraction method has been developed and applied to the samples within this dataset, it is Highly recommended to make the SOP available and provide the link here.	https://github.com/FAIR-eDNA/FAIRe_checklist	according to manufacturer instructions	according to manufacturer instructions			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information associated with the samp_name	https://github.com/FAIR-eDNA/FAIRe_checklist	SAMN51253811	SAMN51253811			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	A raw sequencing data file name of reverse reads if lib_layout = paired end	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_8_10m_01_12S_R2.fq.gz	015_Win_8_10m_01_12S_R2.fq.gz			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Amount or size of original sample (volume, mass or area) that was collected from the environment. Specify the unit under the term samp_size_unit. Note: the amount or size of (sub)sample processed for DNA extraction should be entered under the term samp_vol_we_dna_ext.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	An identifier for the physical sample (as opposed to the extracted DNA sample defined by samp_name). In the absence of a persistent global unique identifier, construct one from a combination of identifiers in the record.	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_8_10m	015_Win_8_10m			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	An identifier of a library (an amplicon of a sample with unique MIDs)	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_8_10m_01_MiFish	015_Win_8_10m_01_MiFish			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Any processing applied to the sample during or after retrieving the sample from environment (i.e. sieving, filtration, precipitation, homogenising, subsampling).	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Chlorophyll fluorescence	http://vocab.nerc.ac.uk/collection/P01/current/CPHLMOD2/	1.771428571	1.771428571	micrograms per litre	http://vocab.nerc.ac.uk/collection/P06/current/UGPL/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of ammonia in the sample	https://vocab.nerc.ac.uk/collection/C77/current/H76/	0.059	0.059	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of dissolved oxygen	https://w3id.org/mixs/0000119	5.53947	5.53947	mg/L	http://vocab.nerc.ac.uk/collection/P06/current/UMGL/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of nitrate in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	1.896	1.896	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of nitrite in the sample	https://github.com/FAIR-eDNA/FAIRe_checklist	0.092	0.092	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of phosphate in the sample	https://w3id.org/mixs/0000505	0.316	0.316	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of silicate in the sample	https://w3id.org/mixs/0000184	2.082	2.082	umol/L	http://vocab.nerc.ac.uk/collection/P06/current/UPOX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Concentration of total DNA after extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	7.3	7.3	nanograms per microlitre		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	DNA concentration method	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit 4.0 Fluorometer (Invitrogen)	Qubit 4.0 Fluorometer (Invitrogen)			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Date of nucleic acid extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	2023-06-27T12:00-07:00	2023-06-27T12:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Description of modification made from a commercial or established protocol (nucl_acid_ext), if made.	https://github.com/FAIR-eDNA/FAIRe_checklist	not applicable	not applicable			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Diameter of water filter	http://vocab.nerc.ac.uk/collection/P01/current/DSAMPA01/	47	47	millimeters	http://vocab.nerc.ac.uk/collection/P06/current/UXMM/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Duration sample was stored prior to DNA extraction	https://github.com/FAIR-eDNA/FAIRe_checklist	P253D	P253D	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Environmental material or materials (pipe separated) immediately surrounded your sample prior to sampling. Select from ENVO's environmental material class (entity/ continuant/ independent continuant/ material entity/ environmental material): http://purl.obolibrary.org/obo/ENVO_00010483. Examples include; soil [ENVO:00001998], marine sediment [ENVO:03000033], air [ENVO:00002005], sea water [ENVO:00002149], fresh water [ENVO:00002011], tap water [ENVO:00003096], fecal material [ENVO:00002003], digestive tract environment [ENVO:2100002], intestine environment [ENVO:2100002], planktonic material [ENVO:01000063]	https://github.com/FAIR-eDNA/FAIRe_checklist	sea water [ENVO:00002149]	sea water [ENVO:00002149]			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Filtering pore size used in sample filtration.	https://github.com/FAIR-eDNA/FAIRe_checklist	0.22	0.22	micrometers		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Forward multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	GGCCAATATT	GGCCAATATT			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Major environmental system your sample came from. The systems identified should have a coarse spatial grain, to provide the general environmental context of where the sampling was done. Select from ENVO's biome class: http://purl.obolibrary.org/obo/ENVO_00000428. Examples include; estuarine biome [ENVO:01000020], tropical moist broadleaf forest biome [ENVO:01000228],	https://github.com/FAIR-eDNA/FAIRe_checklist	marine biome [ENVO:00000447]	marine biome [ENVO:00000447]			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Material of water filter	https://github.com/FAIR-eDNA/FAIRe_checklist	other: polyvinylidene difluoride (PVDF)	other: polyvinylidene difluoride (PVDF)			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Method to estimate lib_conc	https://github.com/FAIR-eDNA/FAIRe_checklist	Qubit Fluorometer	Qubit Fluorometer			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Nucleic acid extraction kit	https://github.com/FAIR-eDNA/FAIRe_checklist	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit	Omega Bio-Tek Mag-Bind® Blood & Tissue DNA HDQ 96 kit			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Nucleic acid extraction lysis approach	https://github.com/FAIR-eDNA/FAIRe_checklist	enzymatic | chemical	enzymatic | chemical			Controlled vocabulary (physical | chemical | enzymatic | thermal | osmotic | other:)
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Nucleic acid extraction separation approach	https://github.com/FAIR-eDNA/FAIRe_checklist	magnetic beads	magnetic beads			Controlled vocabulary (column-based | magnetic beads | centrifugation | precipitation | phenol chloroform | gel electrophoresis | other:)
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	PCR plate ID. This identifier is particularly crucial when location-specific standard curves are employed for targeted assay detections, as it ensures a linkage between the standards and the corresponding eDNA samples. If two-step PCR was applied for a metabarcoding approach, enter the second PCR protocol under the term pcr2_* in the library preparation/sequencing section.	https://github.com/FAIR-eDNA/FAIRe_checklist	GEMCAP/RREAS Plate 1	GEMCAP/RREAS Plate 1			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Refers to the mesh/pore size used to pre-filter/pre-sort the sample. Materials larger than the size threshold are excluded from the sample. Unit = um	https://github.com/FAIR-eDNA/FAIRe_checklist	no pre-filter	no pre-filter			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Reverse multiplex Identifiers (MIDs), that was used to specifically tag unique samples in a sequencing run. Sequence should be reported in uppercase letters	https://github.com/FAIR-eDNA/FAIRe_checklist	TATCGGACCG	TATCGGACCG			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Sample category	https://github.com/FAIR-eDNA/FAIRe_checklist	sample	sample			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Sample collection date before formatting	https://github.com/FAIR-eDNA/FAIRe_checklist	20221017	20221017			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Sample collection time before formatting to ISO 8601, or at the local time (if eventDate time samp has been converted to UTC time)	https://github.com/FAIR-eDNA/FAIRe_checklist	21:00:00	21:00:00			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Solution within which the sample was stored. Note: If a sample was processed before DNA extraction (e.g., filtration, homogenisation, subsampling), enter the storage solution before the process here, and the storage solution of the processed sample under the term prepped_samp_store_sol.	https://w3id.org/mixs/0001317	none	none			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Surface area of a filter.	https://github.com/FAIR-eDNA/FAIRe_checklist	1734.94	1734.94	square millimeters		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Temperature of the sample at the time of sampling in degree Celsius	https://w3id.org/mixs/0000113	16.8276	16.8276	degree Celsius	http://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Temperature sample was stored	https://github.com/FAIR-eDNA/FAIRe_checklist	-80	-80	degree Celsius	https://vocab.nerc.ac.uk/collection/P06/current/UPAA/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The concentration of the prepared library pool that was loaded onto the sequencing platform for a sequencing run.	https://github.com/FAIR-eDNA/FAIRe_checklist	20.0	20.0	nanograms per microlitre		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The date and time of sampling, either as an instance (single point in time) or interval. Must follow ISO 8601 format (yyyy-mm-ddThh:mm:ss). Time zone must be specified after the timestamp (e.g., "2008-01-23T19:23-06:00" in the time zone six hours earlier than UTC, "2008-01-23T19:23Z" at UTC time). In case no exact time is available, the date/time can be right truncated i.e. all of these are valid times: 2008-01-23T19:23:10+00:00; 2008-01-23T19:23:10Z; 2008-01-23; 2008-01; 2008; Except: 2008-01; 2008 all are ISO8601 compliant. A date and time range can be specified using a forward slash (/) to separate the start and end values (e.g., 2008-01-23T19:23-06:00/2008-01-23T19:53-06:00). A duration can also be provided using the eventDurationValue term.	https://github.com/FAIR-eDNA/FAIRe_checklist	2022-10-17T21:00-07:00	2022-10-17T21:00-07:00	ISO 8601	https://vocab.nerc.ac.uk/collection/P06/current/TISO/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The device used to collect an environmental sample. This term accepts terms listed under environmental sampling device (https://ontobee.org/ontology/OBI?iri=http://purl.obolibrary.org/obo/OBI_0000968) such as syringe [OBI_0000422] and container [OBI_0000967] as well as free-text entry (i.e. niskin bottle, push core, spoon, water bottle).	https://github.com/FAIR-eDNA/FAIRe_checklist	Niskin bottle	Niskin bottle			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which coordinates given in verbatimLatitude and verbatimLongitude are based.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The entity or entities which are in your sample or specimen's local vicinity and which you believe have significant causal influences on your sample. Select from ENVO's layer class: http://purl.obolibrary.org/obo/ENVO_01000281. Examples include;  marine pelagic zone [ENVO:00000208], estuarine coastal surface layer [ENVO:01001302], shrub layer [ENVO:01000336]	https://github.com/FAIR-eDNA/FAIRe_checklist	marine pelagic biome [ENVO:01000023]	marine pelagic biome [ENVO:01000023]			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The geographic latitude in decimal degrees using WGS84 datum. Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	36.34642	36.34642		http://rs.tdwg.org/dwc/terms/decimalLatitude	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The geographic longitude in decimal degrees using WGS84 datum. Positive values are east of the Greenwich Meridian, negative values are west of it. Legal values lie between -180 and 180, inclusive. The desired minimum number of decimal places is the hundred-thousandth (0.00001) to achieve meter scale resolution.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867	decimal degrees	http://rs.tdwg.org/dwc/terms/decimalLongitude	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The geographical origin of the sample as defined by the country or sea name followed by regions and localities. Country or sea names should be chosen from the INSDC Geographic Location Name List INSDC country list (http://insdc.org/country.html). Fixed format following <country or sea names>:<region>,<locality>. E.g., "USA: Maryland, Bethesda" , "Atlantic Ocean:Charlie Gibbs Fracture Zone"	https://w3id.org/mixs/0000010	USA: Pacific Ocean, west of California	USA: Pacific Ocean, west of California			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The greater depth of a range of depth (vertical distance below the local surface) at which a sample was collected. For sediment or soil samples depth is measured from sediment or soil surface, respectively. If there is only one single point of depth (no range), the values in minimumDepthInMeters and maximumDepthInMeters are the same. Unit = meter	https://github.com/FAIR-eDNA/FAIRe_checklist	10	10	meters		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The method employed for collecting the sample. Provide a link to a paper/ handbook/ standard operation protocol if any.	https://github.com/FAIR-eDNA/FAIRe_checklist	RREAS - https://zenodo.org/records/15733846	RREAS - https://zenodo.org/records/15733846			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The number of filtered sequence reads for each sample, that made it through bioinformatic filtering and used for final/subsequent analyses. Unit = reads	https://microbiomedata.github.io/nmdc-schema/output_read_count/	9	9	reads		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The original latitude in any format (i.e. deg/min/sec, UTM) before converting to decimal degrees and WGS84. verbatimCoordinateSystem and verbatimSRS should be stored as well.	https://github.com/FAIR-eDNA/FAIRe_checklist	-122.3103867	-122.3103867			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The total concentration of all dissolved salts in a liquid or solid sample. While salinity can be measured by a complete chemical analysis, this method is difficult and time consuming. More often, it is instead derived from the conductivity measurement. This is known as practical salinity. These derivations compare the specific conductance of the sample to a salinity standard such as seawater. Unit = practical salinity unit (psu)	https://w3id.org/mixs/0000183	33.3957	33.3957	practical salinity unit (psu)	http://vocab.nerc.ac.uk/collection/P06/current/PSUX/	
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	The total number of raw sequence reads for each sample. Unit = reads	https://w3id.org/nmdc/input_read_count	38534	38534	reads		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	This term lists the samp_name of biological replicates associated with the current sample. For example, if three biological replicates ("S01_1", "S01_2", and "S01_3") were collected from the same sampling site, the value for biological_rep_relation in each of these replicates would be "S01_1 | S01_2 | S01_3".	https://github.com/FAIR-eDNA/FAIRe_checklist	015_Win_8_10m_01 | 015_Win_8_10m_02 | 015_Win_8_10m_03	015_Win_8_10m_01 | 015_Win_8_10m_02 | 015_Win_8_10m_03	decimal degrees		
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Total number of OTUs or ASVs assigned to taxa based on the study-specific thresholds for each sample	https://github.com/FAIR-eDNA/FAIRe_checklist	9	9			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	User-defined field; CalCOFI-specific station where CTD was cast		68.1 55.9	68.1 55.9			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	User-defined field; Observational notes related to sample collection		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	User-defined field; Observational notes related to sample processing		missing: not collected	missing: not collected			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	User-defined field; Unique sample name according to sequencing run manifest		GEM_R_MiFish_062	GEM_R_MiFish_062			
CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	CalCOFI_Intercal:MiFish:015_Win_8_10m_01_MiFish_occ_ac929cb98b82b3e02d005098b5c25063ab76f72f	Volume or mass of sample or subsamples that was processed for DNA extraction. For example, if 1L of water was collected and filtered, and half of the filter paper was used for DNA extraction, the entries of samp_size and samp_vol_we_dna_ext should be 1L and 500mL respectively. Specify the unit under the term samp_vol_ext_unit.	https://github.com/FAIR-eDNA/FAIRe_checklist	2000	2000	millilitres	https://vocab.nerc.ac.uk/collection/P06/current/VVML/	
