a802e813-af22-4047-a7cd-0b9ec9961ce4 https://ipt-obis.gbif.us/resource?r=biodiversityinformation_2023_oysterreefs_la_mbon biodiversityinformation_2023_oysterreefs_la_mbon Dr. Stephanie Archer Louisiana Universities Marine Consortium Assistant Professor

Chauvin LA 70344 US

sarcher@lumcon.edu 0000-0002-1476-607X Dr. Stephanie Archer Louisiana Universities Marine Consortium Assistant Professor

Chauvin LA 70344 US

sarcher@lumcon.edu 0000-0002-1476-607X Felimon Gayanilo GCOOS Information Systems Architect

6300 Ocean Dr Corpus Christi TX 78412 US

felimon.gayanilo@tamucc.edu 0000-0003-0520-5737 processor Mathew Biddle United States Marine Biodiversity Observation Network (US MBON) Physical Scientist

1315 East-West Highway Silver Spring MD 20910 US

mathew.biddle@noaa.gov https://ioos.noaa.gov/ 0000-0003-4897-1669 distributor 2026-05-26 eng This dataset contains abundance and biomass measurements of oyster-reef associated benthic biodiversity as well as extended measures of oyster reef health including sediment organic matter content, volume of reef material, volume and/or counts of live oysters, mussels, and barnacles, and oyster size data. This dataset is also linked with passive acoustic recordings. occurrence oyster louisiana oyster reef benthic reef-associated habitat trays estuarine GBIF Dataset Type Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_type_2015-07-10.xml observation oyster louisiana oyster reef benthic reef-associated habitat trays estuarine GBIF Dataset Subtype Vocabulary: http://rs.gbif.org/vocabulary/gbif/dataset_subtype.xml This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License. Creative Commons Attribution 4.0 International https://spdx.org/licenses/CC-BY-4.0.html CC-BY-4.0 Gulf of America -90.924 -90.017 29.636 29.061 Assess Louisiana oyster reef-associated benthic biodiversity. This project will establish a new MBON effort in coastal Louisiana, focusing on highly productive and diverse estuarine ecosystems as sentinels of sea-level rise. The project is designed to support ongoing coastal and estuarine monitoring. This dataset contains abundance and biomass measurements of oyster-reef associated benthic biodiversity, as well as extended measures of oyster reef health, including sediment organic matter content, volume of reef material, volume and/or counts of live oysters, mussels, and barnacles, and oyster size data. This dataset is also linked with passive acoustic recordings. This project is funded through the National Aeronautics and Space Administration Award 80NSSC23K0165 NA asNeeded Dr. Stephanie Archer Louisiana Universities Marine Consortium Assistant Professor

Chauvin LA 70344 US

sarcher@lumcon.edu 0000-0002-1476-607X At each oyster reef sediment organic matter and reef material are quantified within 6 haphazardly placed 0.25 x 0.25 m quadrats. Within each quadrat, sediment for organic matter quantification is collected by pushing a 50ml core into the sediment. The core contents are then emptied into a pre-labeled whirl-pak. In the lab, organic matter is quantified using the Loss-on-Ignition method. Next, all reef material within the quadrat up to 10cm depth is collected and rinsed of excess mud. Reef material (live and dead shell material) volume is quantified using the volume displacement method and live oysters and mussels are counted. The first ten live oysters are measured for shell height from umbo to tip. Barnacles are recorded on a relative abundance scale: 0 – no live barnacles; 1 – 1-10 live barnacles; 2 – 11-50 live barnacles; 3 – > 50 live barnacles. The contents of the reef material sampling are added to pre-filled benthic habitat trays so that the contents of one quadrat go into one tray. The benthic habitat trays (n=6, 0.48 × 0.48 x 0.10 m; 20 L) are lined with 3-mm chicken wire and 1-mm mesh bags and pre-filled with ~3L of disarticulated oyster shell. Once all six trays are topped with reef material they are placed in a row on the oyster reef running parallel to shore. A hydrophone (ST 300HF or ST 600HF) set to record continuously a with a 48 kHz sampling rate and high pre-amp gain is attached to a sandbag and deployed in the center of the row of trays. All sampling gear is left for two weeks to allow the community to develop. After two weeks the habitat trays are collected by cinching closed the mesh bag prior to pulling the tray off of the bottom. Once all trays are collected the mesh bags are tied shut and removed from the trays. Excess mud is rinsed from the bags and all material is rinsed through a 1mm mesh sieve. Larger shells are removed and all material retained on the mesh is frozen for future analysis. When ready for processing samples are thawed and rinsed through a 1mm sieve. Material retained on the sieve is visually inspected under 10x magnification and all organisms identified are retained and sorted into probable unique taxa groups. Taxa groups are reviewed and identified to the lowest practicable taxonomic unit (typically species). Individuals within taxa groups are counted, weighed, then dried for 48 hours at 60℃, or until a stable dry weight is reached. When new taxa to the dataset taxa are encountered a voucher specimen is collected. The specimen collected as a voucher is placed into 90% ethanol after a wet weight is recorded. This voucher specimen is recorded separately. The voucher specimens are deposited into the LUMCON Natural History Collection so that taxonomic identification can be continually refined it necessary. Post-processing of hydrophone recordings is described elsewhere. Sampling occurs seasonally (4 times a year) in Barataria Bay (n=3 oyster reefs) and Terrebonne Bay (n=3 oyster reefs) in Louisiana. All taxa visible at 10x magnification are identified and quantified. At each oyster reef sediment organic matter and reef material are quantified within 6 haphazardly placed 0.25 x 0.25 m quadrats. Within each quadrat, sediment for organic matter quantification is collected by pushing a 50ml core into the sediment. The core contents are then emptied into a pre-labeled whirl-pak. In the lab, organic matter is quantified using the Loss-on-Ignition method. Next, all reef material within the quadrat up to 10cm depth is collected and rinsed of excess mud. Reef material (live and dead shell material) volume is quantified using the volume displacement method and live oysters and mussels are counted. The first ten live oysters are measured for shell height from umbo to tip. Barnacles are recorded on a relative abundance scale: 0 – no live barnacles; 1 – 1-10 live barnacles; 2 – 11-50 live barnacles; 3 – > 50 live barnacles. The contents of the reef material sampling are added to pre-filled benthic habitat trays so that the contents of one quadrat go into one tray. The benthic habitat trays (n=6, 0.48 × 0.48 x 0.10 m; 20 L) are lined with 3-mm chicken wire and 1-mm mesh bags and pre-filled with ~3L of disarticulated oyster shell. Once all six trays are topped with reef material they are placed in a row on the oyster reef running parallel to shore. A hydrophone (ST 300HF or ST 600HF) set to record continuously a with a 48 kHz sampling rate and high pre-amp gain is attached to a sandbag and deployed in the center of the row of trays. All sampling gear is left for two weeks to allow the community to develop. After two weeks the habitat trays are collected by cinching closed the mesh bag prior to pulling the tray off of the bottom. Once all trays are collected the mesh bags are tied shut and removed from the trays. Excess mud is rinsed from the bags and all material is rinsed through a 1mm mesh sieve. Larger shells are removed and all material retained on the mesh is frozen for future analysis. When ready for processing samples are thawed and rinsed through a 1mm sieve. Material retained on the sieve is visually inspected under 10x magnification and all organisms identified are retained and sorted into probable unique taxa groups. Taxa groups are reviewed and identified to the lowest practicable taxonomic unit (typically species). Individuals within taxa groups are counted, weighed, then dried for 48 hours at 60℃, or until a stable dry weight is reached. When new taxa to the dataset taxa are encountered a voucher specimen is collected. The specimen collected as a voucher is placed into 90% ethanol after a wet weight is recorded. This voucher specimen is recorded separately. The voucher specimens are deposited into the LUMCON Natural History Collection so that taxonomic identification can be continually refined it necessary. Post-processing of hydrophone recordings is described elsewhere. Voucher specimens serve as a quality control for taxonomic identification. Data entry is checked by a second researcher. Purpose-build code is used to identify unusual data entries. Dr. Stephanie Archer 0000-0002-1476-607X principalInvestigator Granger Hanks 0000-0002-7762-9734 contentProvider Allison Noble 0000-0002-7588-6606 contentProvider Lillian Zhou 0009-0005-1228-5212 contentProvider Katherine Schlachter contentProvider This project will establish a new MBON effort in coastal Louisiana, focusing on highly productive and diverse estuarine ecosystems as sea level rise sentinels NASA 80NSSC23K0165 NASA 80NSSC23K0165 80NSSC23K0165 https://govtribe.com/award/federal-contract-award/grant-for-research-80nssc23k0165?recommendationType=similar_recommendations Louisiana Estuaries (Barataria Bay, Terrebonne Bay, and Atchafalaya Bay) This larger study is aimed at developing remote methods for measuring estuarine biodiversity in low-visibility environments. 2026-04-08T20:31:17.284+00:00 dataset 07/21/2023 - ongoing a802e813-af22-4047-a7cd-0b9ec9961ce4/v1.4.xml