Descrição
These data are amplicon sequence variant (ASV) counts of marine fish derived from environmental DNA (eDNA) sampling targeting the 12S rDNA, V5 region. DNA was extracted from filtered water samples taken off coastal New Jersey during the 2019 New Jersey Ocean Trawl Survey. Sampling corresponded to the New Jersey Ocean Trawl Survey efforts, but trawl data are not included here. The data were originally published as supplementary tables in support of Stoeckle et al. (https://doi.org/10.1093/icesjms/fsaa225). Observations from supplementary tables 8A - 8D were subsequently aligned to Darwin Core.
Registros de Dados
Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 6.868 registros.
Também existem 1 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
Versões
A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.
Como citar
Pesquisadores deveriam citar esta obra da seguinte maneira:
Stoeckle M, Adolf J, Charlop-Powers Z, Dunton K J, Hinks G, VanMorter S M, Formel S K (2023). eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019. Version 1.4. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment&v=1.4
Direitos
Pesquisadores devem respeitar a seguinte declaração de direitos:
O editor e o detentor dos direitos deste trabalho é United States Geological Survey. To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF Registration
Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 46488f96-b51e-4c06-9a02-8c4c900f7f78. United States Geological Survey publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF-US.
Palavras-chave
FISH (UUID: ea855d4c-f132-44f9-b31c-447e1101684d); TRAWL (UUID: c386fdd8-5a49-46c6-9be6-b56a9428f30d); NERITIC ZONE (UUID: eb958dfb-5e38-401f-8b42-5f1273c75a4a); ATLANTIC OCEAN (UUID: cf249a36-2e82-4d32-84cd-23a4f40bb393); NEW JERSEY (UUID: 699b0e5a-6dbd-47aa-b0ca-c4502f044e35); marine biome (ENVO:00000447); coastal water (ENVO:00001250); waterborne particulate matter (ENVO:01000436); marine biota (ENVO: 06105013); Environmental DNA (NCIT:C169196); Fish (NCIT:C14207); Occurrence
Contatos
- Provedor Dos Metadados ●
- Autor ●
- Originador ●
- Ponto De Contato
https://orcid.org/0000-0002-8280-4795
- Autor ●
- Originador
- Autor ●
- Originador ●
- Ponto De Contato
- Autor ●
- Originador
- Provedor Dos Metadados ●
- Processador
Cobertura Geográfica
New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) sites. The neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30m depth contour.
| Coordenadas delimitadoras | Sul Oeste [38,658, -74,969], Norte Leste [40,468, -73,862] |
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Cobertura Taxonômica
Marine Fish
| Class | Teleostei, Elasmobranchii, Chondrostei, Petromyzonti |
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Cobertura Temporal
| Data Inicial / Data final | 2019-01-11 / 2019-11-10 |
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Dados Sobre o Projeto
Nenhuma descrição disponível
| Título | Monmouth University—Rockefeller University Marine Science Policy Initiative |
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O pessoal envolvido no projeto:
Métodos de Amostragem
Samples were taken in the neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30 m depth contour. Prior to tows, a 1.2-l stainless steel, polypropylene-lined Kemmerer water sampler was deployed at surface and bottom, and collected water was transferred to 1-l polypropylene bottles. Once filled, collection bottle was placed inside a plastic bag and into a dedicated cooler kept in a protected cabin location. Samples were maintained on ice and transported to a −20°C freezer within 24 h of collection. Collection bottles were thawed for 24–48 h at 4°C and contents poured into a glass filter manifold attached to wall suction and filtered through a 0.45-μM pore size nitrocellulose filter (Millipore). DNA was extracted with PowerSoil kit (Qiagen) with modifications from the manufacturer’s protocol as previously described.
| Área de Estudo | Sampling of eDNA from filtered seawater taken off the New Jersey coast in 2019 to compare eDNA results to traditional trawl results. Samples were taken for about one-fourth of the trawls conducted by the New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) during 2019. NJ OTS field sampling was conducted during ∼1-week periods in January, June, August, and November 2019. The target for each month was ten paired samples (one surface and one bottom sample per selected tow site) distributed across strata by depth and north-south location. |
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| Controle de Qualidade | As negative controls for each monthly set, 1 l of laboratory tap water was filtered using the same equipment and procedures, and on the same day as for field samples. |
Descrição dos passos do método:
- For more detail see Stoeckle et al., 2020 (https://doi.org/10.1093/icesjms/fsaa225), Stoeckle et al., 2018 (https://doi.org/10.1371/journal.pone.0198717) and Stoeckle et al., 2020 (https://doi.org/10.3389/fmars.2020.00226).
Citações bibliográficas
- Mark Y Stoeckle, Jason Adolf, Zachary Charlop-Powers, Keith J Dunton, Gregory Hinks, Stacy M VanMorter, Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA , ICES Journal of Marine Science, Volume 78, Issue 1, January-February 2021, Pages 293–304, https://doi.org/10.1093/icesjms/fsaa225 https://doi.org/10.1093/icesjms/fsaa225
- Stoeckle M. Y. , Das Mishu M. , Charlop-Powers Z. 2020. Improved environmental DNA reference library identifies overlooked marine fishes in coastal New Jersey, U.S.A. Frontiers in Marine Science, 7: 226 https://doi.org/10.3389/fmars.2020.00226
- Stoeckle, M. Y., Ausubel, J. H., & Coogan, M. (2022). 12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non-fish DNA. Environmental DNA, 00, 1– 11. https://doi.org/10.1002/edn3.376 https://doi.org/10.1002/edn3.376
Metadados Adicionais
| Propósito | Evaluate performance of eDNA for monitoring marine fish populations by comparing bottom trawl catches to eDNA from concurrent water samples. |
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| Identificadores alternativos | https://www.sciencebase.gov/catalog/item/640a18afd34e0e602b90ff45 |
| 46488f96-b51e-4c06-9a02-8c4c900f7f78 | |
| https://obis.org/dataset/fe2ed263-2b21-47d7-a79f-f9b911132398 | |
| https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment |