eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019

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亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

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如何引用

研究者應依照以下指示引用此資源。:

Stoeckle M, Adolf J, Charlop-Powers Z, Dunton K J, Hinks G, VanMorter S M, Formel S K (2023). eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019. Version 1.4. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment&v=1.4

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 United States Geological Survey。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 46488f96-b51e-4c06-9a02-8c4c900f7f78。  United States Geological Survey 發佈此資源，並經由GBIF-US同意向GBIF註冊成為資料發佈者。

關鍵字

FISH (UUID: ea855d4c-f132-44f9-b31c-447e1101684d); TRAWL (UUID: c386fdd8-5a49-46c6-9be6-b56a9428f30d); NERITIC ZONE (UUID: eb958dfb-5e38-401f-8b42-5f1273c75a4a); ATLANTIC OCEAN (UUID: cf249a36-2e82-4d32-84cd-23a4f40bb393); NEW JERSEY (UUID: 699b0e5a-6dbd-47aa-b0ca-c4502f044e35); marine biome (ENVO:00000447); coastal water (ENVO:00001250); waterborne particulate matter (ENVO:01000436); marine biota (ENVO: 06105013); Environmental DNA (NCIT:C169196); Fish (NCIT:C14207); Occurrence

聯絡資訊

地理涵蓋範圍

New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) sites. The neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30m depth contour.

分類群涵蓋範圍

Marine Fish

時間涵蓋範圍

計畫資料

無相關描述

參與計畫的人員:

取樣方法

Samples were taken in the neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30 m depth contour. Prior to tows, a 1.2-l stainless steel, polypropylene-lined Kemmerer water sampler was deployed at surface and bottom, and collected water was transferred to 1-l polypropylene bottles. Once filled, collection bottle was placed inside a plastic bag and into a dedicated cooler kept in a protected cabin location. Samples were maintained on ice and transported to a −20°C freezer within 24 h of collection. Collection bottles were thawed for 24–48 h at 4°C and contents poured into a glass filter manifold attached to wall suction and filtered through a 0.45-μM pore size nitrocellulose filter (Millipore). DNA was extracted with PowerSoil kit (Qiagen) with modifications from the manufacturer’s protocol as previously described.

方法步驟描述:

1. For more detail see Stoeckle et al., 2020 (https://doi.org/10.1093/icesjms/fsaa225), Stoeckle et al., 2018 (https://doi.org/10.1371/journal.pone.0198717) and Stoeckle et al., 2020 (https://doi.org/10.3389/fmars.2020.00226).

引用文獻

1. Mark Y Stoeckle, Jason Adolf, Zachary Charlop-Powers, Keith J Dunton, Gregory Hinks, Stacy M VanMorter, Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA , ICES Journal of Marine Science, Volume 78, Issue 1, January-February 2021, Pages 293–304, https://doi.org/10.1093/icesjms/fsaa225 https://doi.org/10.1093/icesjms/fsaa225
2. Stoeckle M. Y. , Das Mishu M. , Charlop-Powers Z. 2020. Improved environmental DNA reference library identifies overlooked marine fishes in coastal New Jersey, U.S.A. Frontiers in Marine Science, 7: 226 https://doi.org/10.3389/fmars.2020.00226
3. Stoeckle, M. Y., Ausubel, J. H., & Coogan, M. (2022). 12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non-fish DNA. Environmental DNA, 00, 1– 11. https://doi.org/10.1002/edn3.376 https://doi.org/10.1002/edn3.376

額外的詮釋資料