說明
These data are amplicon sequence variant (ASV) counts of marine fish derived from environmental DNA (eDNA) sampling targeting the 12S rDNA, V5 region. DNA was extracted from filtered water samples taken off coastal New Jersey during the 2019 New Jersey Ocean Trawl Survey. Sampling corresponded to the New Jersey Ocean Trawl Survey efforts, but trawl data are not included here. The data were originally published as supplementary tables in support of Stoeckle et al. (https://doi.org/10.1093/icesjms/fsaa225). Observations from supplementary tables 8A - 8D were subsequently aligned to Darwin Core.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 6,868 筆紀錄。
亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本，以便利追蹤其隨時間的變更。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Stoeckle M, Adolf J, Charlop-Powers Z, Dunton K J, Hinks G, VanMorter S M, Formel S K (2023). eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019. Version 1.4. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment&v=1.4
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 United States Geological Survey。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 46488f96-b51e-4c06-9a02-8c4c900f7f78。 United States Geological Survey 發佈此資源，並經由GBIF-US同意向GBIF註冊成為資料發佈者。
關鍵字
FISH (UUID: ea855d4c-f132-44f9-b31c-447e1101684d); TRAWL (UUID: c386fdd8-5a49-46c6-9be6-b56a9428f30d); NERITIC ZONE (UUID: eb958dfb-5e38-401f-8b42-5f1273c75a4a); ATLANTIC OCEAN (UUID: cf249a36-2e82-4d32-84cd-23a4f40bb393); NEW JERSEY (UUID: 699b0e5a-6dbd-47aa-b0ca-c4502f044e35); marine biome (ENVO:00000447); coastal water (ENVO:00001250); waterborne particulate matter (ENVO:01000436); marine biota (ENVO: 06105013); Environmental DNA (NCIT:C169196); Fish (NCIT:C14207); Occurrence
聯絡資訊
- 元數據提供者 ●
- 作者 ●
- 出處 ●
- 連絡人
https://orcid.org/0000-0002-8280-4795
- 作者 ●
- 出處 ●
- 連絡人
- 作者 ●
- 出處
- 元數據提供者 ●
- 處理者
地理涵蓋範圍
New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) sites. The neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30m depth contour.
| 界定座標範圍 | 緯度南界 經度西界 [38.658, -74.969], 緯度北界 經度東界 [40.468, -73.862] |
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分類群涵蓋範圍
Marine Fish
| Class | Teleostei, Elasmobranchii, Chondrostei, Petromyzonti |
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時間涵蓋範圍
| 起始日期 / 結束日期 | 2019-01-11 / 2019-11-10 |
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計畫資料
無相關描述
| 計畫名稱 | Monmouth University—Rockefeller University Marine Science Policy Initiative |
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參與計畫的人員:
取樣方法
Samples were taken in the neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30 m depth contour. Prior to tows, a 1.2-l stainless steel, polypropylene-lined Kemmerer water sampler was deployed at surface and bottom, and collected water was transferred to 1-l polypropylene bottles. Once filled, collection bottle was placed inside a plastic bag and into a dedicated cooler kept in a protected cabin location. Samples were maintained on ice and transported to a −20°C freezer within 24 h of collection. Collection bottles were thawed for 24–48 h at 4°C and contents poured into a glass filter manifold attached to wall suction and filtered through a 0.45-μM pore size nitrocellulose filter (Millipore). DNA was extracted with PowerSoil kit (Qiagen) with modifications from the manufacturer’s protocol as previously described.
| 研究範圍 | Sampling of eDNA from filtered seawater taken off the New Jersey coast in 2019 to compare eDNA results to traditional trawl results. Samples were taken for about one-fourth of the trawls conducted by the New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) during 2019. NJ OTS field sampling was conducted during ∼1-week periods in January, June, August, and November 2019. The target for each month was ten paired samples (one surface and one bottom sample per selected tow site) distributed across strata by depth and north-south location. |
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| 品質控管 | As negative controls for each monthly set, 1 l of laboratory tap water was filtered using the same equipment and procedures, and on the same day as for field samples. |
方法步驟描述:
- For more detail see Stoeckle et al., 2020 (https://doi.org/10.1093/icesjms/fsaa225), Stoeckle et al., 2018 (https://doi.org/10.1371/journal.pone.0198717) and Stoeckle et al., 2020 (https://doi.org/10.3389/fmars.2020.00226).
引用文獻
- Mark Y Stoeckle, Jason Adolf, Zachary Charlop-Powers, Keith J Dunton, Gregory Hinks, Stacy M VanMorter, Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA , ICES Journal of Marine Science, Volume 78, Issue 1, January-February 2021, Pages 293–304, https://doi.org/10.1093/icesjms/fsaa225 https://doi.org/10.1093/icesjms/fsaa225
- Stoeckle M. Y. , Das Mishu M. , Charlop-Powers Z. 2020. Improved environmental DNA reference library identifies overlooked marine fishes in coastal New Jersey, U.S.A. Frontiers in Marine Science, 7: 226 https://doi.org/10.3389/fmars.2020.00226
- Stoeckle, M. Y., Ausubel, J. H., & Coogan, M. (2022). 12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non-fish DNA. Environmental DNA, 00, 1– 11. https://doi.org/10.1002/edn3.376 https://doi.org/10.1002/edn3.376
額外的詮釋資料
| 目的 | Evaluate performance of eDNA for monitoring marine fish populations by comparing bottom trawl catches to eDNA from concurrent water samples. |
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| 替代的識別碼 | https://www.sciencebase.gov/catalog/item/640a18afd34e0e602b90ff45 |
| 46488f96-b51e-4c06-9a02-8c4c900f7f78 | |
| https://obis.org/dataset/fe2ed263-2b21-47d7-a79f-f9b911132398 | |
| https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment |