Descripción
These data are amplicon sequence variant (ASV) counts of marine fish derived from environmental DNA (eDNA) sampling targeting the 12S rDNA, V5 region. DNA was extracted from filtered water samples taken off coastal New Jersey during the 2019 New Jersey Ocean Trawl Survey. Sampling corresponded to the New Jersey Ocean Trawl Survey efforts, but trawl data are not included here. The data were originally published as supplementary tables in support of Stoeckle et al. (https://doi.org/10.1093/icesjms/fsaa225). Observations from supplementary tables 8A - 8D were subsequently aligned to Darwin Core.
Registros
Los datos en este recurso de registros biológicos han sido publicados como Archivo Darwin Core(DwC-A), el cual es un formato estándar para compartir datos de biodiversidad como un conjunto de una o más tablas de datos. La tabla de datos del core contiene 6.868 registros.
también existen 1 tablas de datos de extensiones. Un registro en una extensión provee información adicional sobre un registro en el core. El número de registros en cada tabla de datos de la extensión se ilustra a continuación.
Este IPT archiva los datos y, por lo tanto, sirve como repositorio de datos. Los datos y los metadatos del recurso están disponibles para su descarga en la sección descargas. La tabla versiones enumera otras versiones del recurso que se han puesto a disposición del público y permite seguir los cambios realizados en el recurso a lo largo del tiempo.
Versiones
La siguiente tabla muestra sólo las versiones publicadas del recurso que son de acceso público.
¿Cómo referenciar?
Los usuarios deben citar este trabajo de la siguiente manera:
Stoeckle M, Adolf J, Charlop-Powers Z, Dunton K J, Hinks G, VanMorter S M, Formel S K (2023). eDNA observations, concurrent with trawl survey, of marine fish in coastal New Jersey, USA 2019. Version 1.4. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment&v=1.4
Derechos
Los usuarios deben respetar los siguientes derechos de uso:
El publicador y propietario de los derechos de este trabajo es United States Geological Survey. En la medida de lo posible según la ley, el publicador ha renunciado a todos los derechos sobre estos datos y los ha dedicado al Dominio público (CC0 1.0). Los usuarios pueden copiar, modificar, distribuir y utilizar la obra, incluso con fines comerciales, sin restricciones.
Registro GBIF
Este recurso ha sido registrado en GBIF con el siguiente UUID: 46488f96-b51e-4c06-9a02-8c4c900f7f78. United States Geological Survey publica este recurso y está registrado en GBIF como un publicador de datos avalado por GBIF-US.
Palabras clave
FISH (UUID: ea855d4c-f132-44f9-b31c-447e1101684d); TRAWL (UUID: c386fdd8-5a49-46c6-9be6-b56a9428f30d); NERITIC ZONE (UUID: eb958dfb-5e38-401f-8b42-5f1273c75a4a); ATLANTIC OCEAN (UUID: cf249a36-2e82-4d32-84cd-23a4f40bb393); NEW JERSEY (UUID: 699b0e5a-6dbd-47aa-b0ca-c4502f044e35); marine biome (ENVO:00000447); coastal water (ENVO:00001250); waterborne particulate matter (ENVO:01000436); marine biota (ENVO: 06105013); Environmental DNA (NCIT:C169196); Fish (NCIT:C14207); Occurrence
Contactos
- Proveedor De Los Metadatos ●
- Autor ●
- Originador ●
- Punto De Contacto
- Senior Research Associate
https://orcid.org/0000-0002-8280-4795
- Autor ●
- Originador
- Professor
- Autor ●
- Originador ●
- Punto De Contacto
- Fisheries Biologist
- Autor ●
- Originador
- Research Technician
- Proveedor De Los Metadatos ●
- Procesador
- Biologist
Cobertura geográfica
New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) sites. The neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30m depth contour.
| Coordenadas límite | Latitud Mínima Longitud Mínima [38,658, -74,969], Latitud Máxima Longitud Máxima [40,468, -73,862] |
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Cobertura taxonómica
Marine Fish
| Class | Teleostei, Elasmobranchii, Chondrostei, Petromyzonti |
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Cobertura temporal
| Fecha Inicial / Fecha Final | 2019-01-11 / 2019-11-10 |
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Datos del proyecto
No hay descripción disponible
| Título | Monmouth University—Rockefeller University Marine Science Policy Initiative |
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Personas asociadas al proyecto:
Métodos de muestreo
Samples were taken in the neritic zone ocean from New York Harbor south to Delaware Bay, from shoreline out to 30 m depth contour. Prior to tows, a 1.2-l stainless steel, polypropylene-lined Kemmerer water sampler was deployed at surface and bottom, and collected water was transferred to 1-l polypropylene bottles. Once filled, collection bottle was placed inside a plastic bag and into a dedicated cooler kept in a protected cabin location. Samples were maintained on ice and transported to a −20°C freezer within 24 h of collection. Collection bottles were thawed for 24–48 h at 4°C and contents poured into a glass filter manifold attached to wall suction and filtered through a 0.45-μM pore size nitrocellulose filter (Millipore). DNA was extracted with PowerSoil kit (Qiagen) with modifications from the manufacturer’s protocol as previously described.
| Área de Estudio | Sampling of eDNA from filtered seawater taken off the New Jersey coast in 2019 to compare eDNA results to traditional trawl results. Samples were taken for about one-fourth of the trawls conducted by the New Jersey Bureau of Marine Fisheries Ocean Trawl Survey (NJ OTS) during 2019. NJ OTS field sampling was conducted during ∼1-week periods in January, June, August, and November 2019. The target for each month was ten paired samples (one surface and one bottom sample per selected tow site) distributed across strata by depth and north-south location. |
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| Control de Calidad | As negative controls for each monthly set, 1 l of laboratory tap water was filtered using the same equipment and procedures, and on the same day as for field samples. |
Descripción de la metodología paso a paso:
- For more detail see Stoeckle et al., 2020 (https://doi.org/10.1093/icesjms/fsaa225), Stoeckle et al., 2018 (https://doi.org/10.1371/journal.pone.0198717) and Stoeckle et al., 2020 (https://doi.org/10.3389/fmars.2020.00226).
Referencias bibliográficas
- Mark Y Stoeckle, Jason Adolf, Zachary Charlop-Powers, Keith J Dunton, Gregory Hinks, Stacy M VanMorter, Trawl and eDNA assessment of marine fish diversity, seasonality, and relative abundance in coastal New Jersey, USA , ICES Journal of Marine Science, Volume 78, Issue 1, January-February 2021, Pages 293–304, https://doi.org/10.1093/icesjms/fsaa225 https://doi.org/10.1093/icesjms/fsaa225
- Stoeckle M. Y. , Das Mishu M. , Charlop-Powers Z. 2020. Improved environmental DNA reference library identifies overlooked marine fishes in coastal New Jersey, U.S.A. Frontiers in Marine Science, 7: 226 https://doi.org/10.3389/fmars.2020.00226
- Stoeckle, M. Y., Ausubel, J. H., & Coogan, M. (2022). 12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non-fish DNA. Environmental DNA, 00, 1– 11. https://doi.org/10.1002/edn3.376 https://doi.org/10.1002/edn3.376
Metadatos adicionales
| Propósito | Evaluate performance of eDNA for monitoring marine fish populations by comparing bottom trawl catches to eDNA from concurrent water samples. |
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| Identificadores alternativos | https://www.sciencebase.gov/catalog/item/640a18afd34e0e602b90ff45 |
| 46488f96-b51e-4c06-9a02-8c4c900f7f78 | |
| https://obis.org/dataset/fe2ed263-2b21-47d7-a79f-f9b911132398 | |
| https://ipt-obis.gbif.us/resource?r=rockefeller_njtrawl_edna_assessment |