Marine zooplankton community structure through environmental DNA metabarcoding in the Florida Keys, collected by the South Florida Program (NOAA/AOML) and the Marine Biodiversity Observation Network (MBON)

Ocorrência
Versão mais recente published by United States Geological Survey on nov 18, 2023 United States Geological Survey
Publication date:
18 de novembro de 2023
Licença:
CC0 1.0

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Descrição

This data consists of counts of putative zooplankton OTUs, revealed through metabarcoding of the cytochrome oxidase I (COI) gene extracted from filtered water samples in the Florida Keys National Marine Sanctuary during 2016. It includes results from 95 water samples taken over three days at three coral reef sites. After data processing, a total of 194 taxa were identified; the identification was to the species level, with only a few to the genus level.

Registros de Dados

Os dados deste recurso de ocorrência foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 3.123 registros.

Também existem 1 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.

Occurrence (core)
3123
dnaDerivedData 
3123

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Como citar

Pesquisadores deveriam citar esta obra da seguinte maneira:

Djurhuus A, Peralta A C, Pitz K, Sawaya N A, Rojas-Marquez J, Michaud B, Montes E, Muller-Karger F, Breitbart M (2023). Marine zooplankton community structure through environmental DNA metabarcoding in the Florida Keys, collected by the South Florida Program (NOAA/AOML) and the Marine Biodiversity Observation Network (MBON). Version 1.3. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=mbonfknmsedna&v=1.3

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é United States Geological Survey. To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 9d6c78cc-74c6-4a12-b65b-0c9254ddb0b6.  United States Geological Survey publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF-US.

Palavras-chave

Occurrence; zooplankton; DNA sequencing; molecular taxonomy; biodiversity; ocean environment; Occurrence; Observation

Contatos

Anni Djurhuus
  • Provedor Dos Metadados ●
  • Originador ●
  • Ponto De Contato
  • postdoctoral researcher
College of Marine Science, University of South Florida
US
Ana Carolina Peralta
  • Provedor Dos Metadados ●
  • Originador ●
  • Ponto De Contato
  • postdoctoral researcher
College of Marine Science, University of South Florida
US
Kathleen Pitz
  • Originador
  • Researcher
Monterey Bay Aquatic Research Institute
California
US
Natalie A. Sawaya
  • Originador
  • Researcher
College of Marine Science, University of South Florida
US
Jaimie Rojas-Marquez
  • Originador
  • Researcher
Fundacion La Salle de Ciencias Naturales, Estacion de Investigaciones Marinas
Margarita
VE
Brianna Michaud
  • Originador
  • Researcher
College of Marine Science, University of South Florida
US
Enrique Montes
  • Originador
  • NOAA Affiliated Researcher
Cooperative Institute for Marine & Atmospheric Studies (CIMAS)
US
Frank Muller-Karger
  • Originador ●
  • Ponto De Contato
  • Project Principal Investigator
College of Marine Science, University of South Florida
US
Mya Breitbart
  • Originador
  • Professor and researcher
College of Marine Science, University of South Florida
US
Stephen Formel
  • Verificador
  • Biologist
U.S. Geological Survey
Mathew Biddle
  • Distribuidor
  • Physical Scientist
United States Marine Biodiversity Observation Network (US MBON)
  • 1315 East-West Highway
20910 Silver Spring
MD
US
  • 3017134928

Cobertura Geográfica

Florida Keys: Molasses Reef, Looe Key, and Western Sambo

Coordenadas delimitadoras Sul Oeste [24,286, -81,654], Norte Leste [25,352, -80,228]

Cobertura Taxonômica

Zooplankton, including 194 taxa within 37 Classes.

Class Coccolithophyceae, Copepoda, Teleostei, Ascidiacea, Hydrozoa, Sagittoidea, Malacostraca, Gastropoda, Mamiellophyceae, Eurotiomycetes, Chlorarachnea, Bacillariophyceae, Polychaeta, Ostracoda, Palaeonemertea, Anthozoa, Cephalopoda, Pilidiophora, Demospongiae, Ophiuroidea, Thecostraca, Florideophyceae, Cyanophyceae, Holothuroidea, Pyramimonadophyceae, Hexapoda, Homoscleromorpha, Bivalvia, Elasmobranchii, Gymnolaemata, Asteroidea, Dinophyceae, Cryptophyta incertae sedis, Raphidophyce, Echinoidea, Aves, Nuda

Cobertura Temporal

Data Inicial / Data final 2016-03-15 / 2016-05-16

Dados Sobre o Projeto

A close partnership with NOAA AOML and the FKNMS has focused on periodic MBON surveys of the Florida Keys since 2014. Additional partners now add animal tracking and other dimensions to the Sanctuaries MBON. Specifically, we seek to integrate ground and satellite observations related to biodiversity to inform ecosystem-based management in and around the Florida Keys National Marine Sanctuary (FKNMS). This includes supporting the regional NOAA Integrated Ecosystem Assessment (IEA), the National Marine Fisheries Southeast Fisheries Science Center, the FKNMS, Rookery Bay National Estuarine Research Reserve (NERR), and the State of Florida Fish and Wildlife Commission (FWC) as specific end users of an MBON.

Título South Florida Program (NOAA – AOML) and Marine Biodiversity Observation Network (MBON)
Financiamento This work is a contribution to the Marine Biodiversity Observation Network (MBON). The MBON project was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a Demonstration Marine Biodiversity Observation Network (MBON)’ funded under the National Ocean Partnership Programme (NOPP RFP NOAA-NOS-IOOS-2014-2003803 in partnership between NOAA, BOEM and NASA), and the U.S. Integrated Ocean Observing System (IOOS) Programme Office.

O pessoal envolvido no projeto:

Frank Muller-Karger
  • Pesquisador Principal

Métodos de Amostragem

This data represents zooplankton taxonomic composition revealed through metabarcoding of the cytochrome oxidase I (COI) genes. Water samples were collected from three coral reef sites in the Florida Keys National Marine Sanctuary. 1 L surface seawater samples prefiltered through 3 um filters and subsequently collected on 0.22 um filters for eDNA (PF-eDNA). The COI gene metabarcoding of PF-eDNA samples was performed using Illumina MiSeq.

Área de Estudo Sampling area: Florida Keys - Molasses Reef, Looe Key, and Western Sambo.

Descrição dos passos do método:

  1. https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lom3.10237

Metadados Adicionais

Propósito

MBON cruises have the purpose of collection biological data for biodiversity assessments. Water samples were used to determine dominant copepod survey using eDNA, demonstrating that eDNA metabarcoding is a promising technique for future biodiversity assessments of pelagic zooplankton in marine systems.

Identificadores alternativos 9d6c78cc-74c6-4a12-b65b-0c9254ddb0b6
https://ipt-obis.gbif.us/resource?r=mbonfknmsedna