Marine zooplankton community structure through environmental DNA metabarcoding in the Florida Keys, collected by the South Florida Program (NOAA/AOML) and the Marine Biodiversity Observation Network (MBON)

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說明

This data consists of counts of putative zooplankton OTUs, revealed through metabarcoding of the cytochrome oxidase I (COI) gene extracted from filtered water samples in the Florida Keys National Marine Sanctuary during 2016. It includes results from 95 water samples taken over three days at three coral reef sites. After data processing, a total of 194 taxa were identified; the identification was to the species level, with only a few to the genus level.

資料紀錄

此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。核心資料表包含 3,123 筆紀錄。

亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。每個延伸集資料表中資料筆數顯示如下。

3123

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如何引用

研究者應依照以下指示引用此資源。:

Djurhuus A, Peralta A C, Pitz K, Sawaya N A, Rojas-Marquez J, Michaud B, Montes E, Muller-Karger F, Breitbart M (2023). Marine zooplankton community structure through environmental DNA metabarcoding in the Florida Keys, collected by the South Florida Program (NOAA/AOML) and the Marine Biodiversity Observation Network (MBON). Version 1.3. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=mbonfknmsedna&v=1.3

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 United States Geological Survey。 To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.

GBIF 註冊

此資源已向GBIF註冊，並指定以下之GBIF UUID: 9d6c78cc-74c6-4a12-b65b-0c9254ddb0b6。 United States Geological Survey 發佈此資源，並經由GBIF-US同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; zooplankton; DNA sequencing; molecular taxonomy; biodiversity; ocean environment; Occurrence; Observation

聯絡資訊

Anni Djurhuus

元數據提供者 ●
出處 ●
連絡人

postdoctoral researcher

College of Marine Science, University of South Florida

anni.djurhuus@gmail.com

https://orcid.org/0000-0002-3517-7522

Ana Carolina Peralta

元數據提供者 ●
出處 ●
連絡人

postdoctoral researcher

College of Marine Science, University of South Florida

anacarolaperaltab@gmail.com

https://orcid.org/0000-0003-0830-9814

Kathleen Pitz

出處

Researcher

Monterey Bay Aquatic Research Institute

California

kpitz@mbari.org

https://orcid.org/0000-0001-6841-2659

Natalie A. Sawaya

出處

Researcher

College of Marine Science, University of South Florida

https://orcid.org/0000-0001-6841-2659

Jaimie Rojas-Marquez

出處

Researcher

Fundacion La Salle de Ciencias Naturales, Estacion de Investigaciones Marinas

Margarita

https://orcid.org/0009-0009-7318-7180

Brianna Michaud

出處

Researcher

College of Marine Science, University of South Florida

bmichaud@usf.edu

https://orcid.org/0000-0002-8653-3603

Enrique Montes

出處

NOAA Affiliated Researcher

Cooperative Institute for Marine & Atmospheric Studies (CIMAS)

enrique.montes@noaa.gov

https://orcid.org/0000-0002-3103-4479

Frank Muller-Karger

出處 ●
連絡人

Project Principal Investigator

College of Marine Science, University of South Florida

carib@usf.edu

https://orcid.org/0000-0003-3159-5011

Mya Breitbart

出處

Professor and researcher

College of Marine Science, University of South Florida

mya@usf.edu

https://orcid.org/0000-0003-3210-2899

Stephen Formel

審閱者

Biologist

U.S. Geological Survey

sformel@usgs.gov

https://orcid.org/0000-0001-7418-1244

Mathew Biddle

散布者

Physical Scientist

United States Marine Biodiversity Observation Network (US MBON)

1315 East-West Highway

20910 Silver Spring

mathew.biddle@noaa.gov

3017134928

https://ioos.noaa.gov/

https://orcid.org/0000-0003-4897-1669

地理涵蓋範圍

Florida Keys: Molasses Reef, Looe Key, and Western Sambo

界定座標範圍	緯度南界經度西界 [24.286, -81.654], 緯度北界經度東界 [25.352, -80.228]

分類群涵蓋範圍

Zooplankton, including 194 taxa within 37 Classes.

Class	Coccolithophyceae, Copepoda, Teleostei, Ascidiacea, Hydrozoa, Sagittoidea, Malacostraca, Gastropoda, Mamiellophyceae, Eurotiomycetes, Chlorarachnea, Bacillariophyceae, Polychaeta, Ostracoda, Palaeonemertea, Anthozoa, Cephalopoda, Pilidiophora, Demospongiae, Ophiuroidea, Thecostraca, Florideophyceae, Cyanophyceae, Holothuroidea, Pyramimonadophyceae, Hexapoda, Homoscleromorpha, Bivalvia, Elasmobranchii, Gymnolaemata, Asteroidea, Dinophyceae, Cryptophyta incertae sedis, Raphidophyce, Echinoidea, Aves, Nuda

Class

Coccolithophyceae, Copepoda, Teleostei, Ascidiacea, Hydrozoa, Sagittoidea, Malacostraca, Gastropoda, Mamiellophyceae, Eurotiomycetes, Chlorarachnea, Bacillariophyceae, Polychaeta, Ostracoda, Palaeonemertea, Anthozoa, Cephalopoda, Pilidiophora, Demospongiae, Ophiuroidea, Thecostraca, Florideophyceae, Cyanophyceae, Holothuroidea, Pyramimonadophyceae, Hexapoda, Homoscleromorpha, Bivalvia, Elasmobranchii, Gymnolaemata, Asteroidea, Dinophyceae, Cryptophyta incertae sedis, Raphidophyce, Echinoidea, Aves, Nuda

時間涵蓋範圍

起始日期 / 結束日期	2016-03-15 / 2016-05-16

計畫資料

A close partnership with NOAA AOML and the FKNMS has focused on periodic MBON surveys of the Florida Keys since 2014. Additional partners now add animal tracking and other dimensions to the Sanctuaries MBON. Specifically, we seek to integrate ground and satellite observations related to biodiversity to inform ecosystem-based management in and around the Florida Keys National Marine Sanctuary (FKNMS). This includes supporting the regional NOAA Integrated Ecosystem Assessment (IEA), the National Marine Fisheries Southeast Fisheries Science Center, the FKNMS, Rookery Bay National Estuarine Research Reserve (NERR), and the State of Florida Fish and Wildlife Commission (FWC) as specific end users of an MBON.

計畫名稱	South Florida Program (NOAA – AOML) and Marine Biodiversity Observation Network (MBON)
經費來源	This work is a contribution to the Marine Biodiversity Observation Network (MBON). The MBON project was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a Demonstration Marine Biodiversity Observation Network (MBON)’ funded under the National Ocean Partnership Programme (NOPP RFP NOAA-NOS-IOOS-2014-2003803 in partnership between NOAA, BOEM and NASA), and the U.S. Integrated Ocean Observing System (IOOS) Programme Office.

計畫名稱

South Florida Program (NOAA – AOML) and Marine Biodiversity Observation Network (MBON)

經費來源

This work is a contribution to the Marine Biodiversity Observation Network (MBON). The MBON project was supported by NASA grant NNX14AP62A ‘National Marine Sanctuaries as Sentinel Sites for a Demonstration Marine Biodiversity Observation Network (MBON)’ funded under the National Ocean Partnership Programme (NOPP RFP NOAA-NOS-IOOS-2014-2003803 in partnership between NOAA, BOEM and NASA), and the U.S. Integrated Ocean Observing System (IOOS) Programme Office.

參與計畫的人員:

Frank Muller-Karger

研究主持人

https://orcid.org/0000-0003-3159-5011

取樣方法

This data represents zooplankton taxonomic composition revealed through metabarcoding of the cytochrome oxidase I (COI) genes. Water samples were collected from three coral reef sites in the Florida Keys National Marine Sanctuary. 1 L surface seawater samples prefiltered through 3 um filters and subsequently collected on 0.22 um filters for eDNA (PF-eDNA). The COI gene metabarcoding of PF-eDNA samples was performed using Illumina MiSeq.

研究範圍	Sampling area: Florida Keys - Molasses Reef, Looe Key, and Western Sambo.

方法步驟描述:

https://aslopubs.onlinelibrary.wiley.com/doi/10.1002/lom3.10237

額外的詮釋資料

目的	MBON cruises have the purpose of collection biological data for biodiversity assessments. Water samples were used to determine dominant copepod survey using eDNA, demonstrating that eDNA metabarcoding is a promising technique for future biodiversity assessments of pelagic zooplankton in marine systems.
替代的識別碼	9d6c78cc-74c6-4a12-b65b-0c9254ddb0b6
	https://ipt-obis.gbif.us/resource?r=mbonfknmsedna