Seawater was collected on board the NOAA ship Ronald H. Brown as part of the fourth Gulf of Mexico Ecosystems and Carbon Cycle (GOMECC-4) cruise from September 13 to October 21, 2021. Sampling for GOMECC-4 occurred along 16 coastal-offshore transects across the entire Gulf of Mexico and an additional line at 27N latitude in the Atlantic Ocean. We also collected eDNA samples near Padre Island National Seashore (U.S. National Parks Service), a barrier island located off the coast of south Texas. Vertical CTD sampling was employed at each site to measure discrete chemical, physical, and biological properties. Water sampling for DNA filtration was conducted at 54 out of 141 total sites and three depths per site (surface, deep chlorophyll maximum, and near bottom) to capture horizontal and vertical gradients of bacterial, protistan, and metazoan diversity across the Gulf.
The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 311,390 records.
1 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.
This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.
The table below shows only published versions of the resource that are publicly accessible.
How to cite
Researchers should cite this work as follows:
Silliman K, Anderson S, Thompson L (2023). eDNA from Gulf of Mexico Ecosystems and Carbon Cruise 2021 (GOMECC-4). Version 1.6. United States Geological Survey. Occurrence dataset. https://ipt-obis.gbif.us/resource?r=noaa-aoml-gomecc4&v=1.6
Researchers should respect the following rights statement:
The publisher and rights holder of this work is United States Geological Survey. To the extent possible under law, the publisher has waived all rights to these data and has dedicated them to the Public Domain (CC0 1.0). Users may copy, modify, distribute and use the work, including for commercial purposes, without restriction.
This resource has been registered with GBIF, and assigned the following GBIF UUID: 9012def0-bd87-48a0-ac9e-e0e78dd37689. United States Geological Survey publishes this resource, and is itself registered in GBIF as a data publisher endorsed by U.S. Geological Survey.
Occurrence; Observation; ATLANTIC OCEAN (UUID: cf249a36-2e82-4d32-84cd-23a4f40bb393); GULF OF MEXICO (UUID: 75fab119-51a6-4b59-9711-f6c3cd3139db); Atlantic Oceanographic and Meteorological Laboratory; OAR; NOAA; U.S. Department of Commerce (UUID: 274cc3ed-e075-4fb3-89cd-51fa72bc64f9)
The resource data is also available in other formats
|Github Code Repo||https://github.com/aomlomics/gomecc Unknown R and Python code|
|NCBI BioProject||https://www.ncbi.nlm.nih.gov/bioproject/PRJNA887898/ Unknown FASTQ|
Gulf of Mexico and south Atlantic Ocean
|Bounding Coordinates||South West [18.836, -97.639], North East [30.111, -79.618]|
18S and 16S sequences assigned to Bacteria, Archaea, Chromista, Animalia, Plantae, Protozoa, and Fungi.
|Kingdom||Bacteria, Archaeae, Chromista, Animalia, Plantae, Protozoa, Fungi|
|Start Date / End Date||2021-09-14 / 2021-10-20|
The effort was in support of the coastal monitoring and research objectives of NOAA’S Ocean Acidification Program. The cruise was designed to obtain a snapshot of key carbon, physical, and biogeochemical parameters as they relate to ocean acidification (OA) in the coastal realm. This was the fourth occupation of the Gulf of Mexico as part of the Ocean Acidification Program’s monitoring efforts, with the first three occurring in 2007, 2012 and 2017.
|Title||Gulf of Mexico Ecosystems and Carbon Cycle|
|Funding||NOAA/OAR Ocean Acidification Program Grant 21392, "Assessing ecosystem responses of Gulf of Mexico communities to OA using environmental DNA (or: Understanding biodiversity in the Gulf of Mexico using eDNA) http://dx.doi.org/10.13039/100018228|
|Study Area Description||This report describes the fourth Gulf of Mexico Ecosystems and Carbon Cycle (GOMECC4) cruise on board the R/V Ronald H. Brown departing from Key West, FL into the Gulf of Mexico and then around the coastal waters of the Gulf of Mexico in a counter-clockwise direction. The cruise took place from September 13–October 21, 2021|
|Design Description||The cruise included a series of 15 transects approximately orthogonal to the coast of the Gulf of Mexico and a 16th partial transect along the 27°N line, between Florida and the Bahamas, as well as a comprehensive set of underway measurements along the entire cruise track. In addition to these transects, three more stations were sampled as part of a collaboration with the National Parks Service to monitor ocean acidification at national parks. Four parks participated in this effort: Padre Island (in Texas), Dry Tortugas, Everglades, and Biscayne (these three in Florida).|
The personnel involved in the project:
For more information see the cruise report: https://doi.org/10.25923/rwx5-s713
|Study Extent||For more information see the cruise report: https://doi.org/10.25923/rwx5-s713|
Method step description:
- Detailed methods will be published in forthcoming paper. This field will be updated after publication.
- Barbero, Leticia et al. "Cruise report: Fourth Gulf of Mexico Ecosystems and Carbon Cycle (GOMECC-4) Cruise", 2021 https://doi.org/10.25923/rwx5-s713
|Purpose||Implement eDNA sampling to better understand the impact of ocean acidification on marine life (bacteria to fish), develop models to predict indicator species and their responses to future climate scenarios, and inform ecosystem management strategies.|